General Kinase Substrate Set

Product Code: KSS-B-720-250

Collection of 720 N-terminally biotinylated peptides of Homo sapiens (Human) selected from the Swiss-Prot and Phosphobase databases.Amount: 2x 384-well MTP (250 pmol per well)Purity: 95% (HPLC-MS) Delivery Format: 384 well microtiter plate Application(s): Phosphosite detection, Substrate identificationIndication(s)/Topic(s): KinaseDelivery Time: 2-5 days

Enzyme Substrate Sets One of the most efficient ways to study enzyme activity and substrate specificity is incubating the enzyme of interest with multiple substrate peptides in parallel. JPT Peptide Technologies ready-to-screen Substrate Sets are comprised of numerous substrate peptides, which were carefully selected from the Swiss-Prot and Phosphobase databases or bioinformatically designed. The Substrate Sets are delivered in microtiter plates for direct incubation with target enyzme.

Benefits of JPTs Enzyme Substrate Sets - Screen numerous potential enzyme substrates simultaneously at low cost - Save time for HTS-Assay development- Learn more about the biology of your enzyme- Read-out with common ELISA readers - Identification of potential in vivo substrates - Determine kinetic constants

General Kinase Substrate Set

Selected References \"Structural Characterization of Zinc-bound Zmp1, a Zinc-dependent Metalloprotease Secreted by Clostridium Difficile\" Rubino et al., J Biol Inorg Chem. (2015) - PMID: 26711661 \"Matrix Metalloproteinase 10 Degradomics in Keratinocytes and Epidermal Tissue Identifies Bioactive Substrates with Pleiotropic Functions\" Schlage et al., Mol Cell Proteomics. (2015) - PMID: 26475864

Testimonials\"We were very pleased with how easy it was to do the experiment (with Kinase Substrate Sets) using the equipment we had on hand - for example, we just used our 8-channel micropipette to add the enzyme/ATP mix.\"Betty A. Eipper, Ph.D, University of Connecticut, Farmington, USA\"Our research relies heavily on developing robust high-throughput screens with fluorescent peptides. We have found that JPTs are the best on the market because the signal-to-noise ratio is very high, providing the sensitivity we need for the screens. Their peptides always perform well.In addition, the knowledge, wonderful customer support, and fast turnaround time provided by JPT have been invaluable in helping us develop the best peptides for our assays.\"Professor Carla Koehler, UCLA, Los Angeles, CA

General Kinase Substrate Set