The EpiQuik™ Total Histone Extraction Kitis a complete set of optimized buffers and reagents for extracting total core histone proteins (H2A, H2B, H3, and H4) from mammalian cells or tissuesin a simple 60 minute procedure.The post-translational modifications (PTM) in the histone extracts are kept intact and thus can be used with Epigentekshistone modificationassay kits or in a variety of downstream applications for histonemethylation, acetylation, phosphorylation,sumoylation, ubiquitination,citrullination, and ADP-ribosylationstudies.

• Pre-optimized and simple 1 hour protocol.
• Conveniently includes all essential reagents to carry out a histone extraction.
• Standardized procedure for reproducible results.
• Extracts a high yield of total core histones from as little as1mg of tissues.
• Post-translational modifications are kept intact.
• Does not affect histone modification status or levels.

Background InformationHistones are the chief protein components of chromatin in biology. They act as spools around which DNA winds, and also play a role in gene regulation. The core histones include H2A, H2B, H3, and H4. Histones undergo post-translational modifications, which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have long tails protruding from the nucleosome, which can be covalently modified at several places (i.e., methylation, acetylation, phosphorylation, etc.). Histone modifications act in diverse biological processes such as gene regulation, DNA repair and chromosome condensation (mitosis). Combinations of modifications are thought to constitute a\"histone code\" and an understanding of this code may help to answer questions about human development and disease.

Principle & ProcedureThe EpiQuik™ Total Histone Extraction Kit simply applies our proprietary histone isolation buffers to cell or tissue samples. After treatment with pre-lysis, lysis, and balance buffers, the total corehistones are easily extracted for immediate use or storage under proper conditions.

Starting MaterialsThe minimal amount of starting materials can be as low as 10⁵cells or 1 mg tissue. For the best results, the cell number should be greater than 10⁶cells or the tissue amount should be greater than 10 mg. A total of 100 standard extractions (using 10⁷cells or 100 mg tissues per extraction) can be performed with this kit. Yield of the total histone proteins is approximately 0.4 mg per 10⁷cells or 100 mg of tissue. The yield may vary depending on cell or tissue type.

10X Pre-Lysis BufferLysis BufferBalance BufferDTT SolutionUser Guide