产品名称 庆大霉素硫酸盐：Gentamicin sulfate salt ——【产品编号：SIGMA-ALDRICH-G3632】 Tips庆大霉素靠结合到30S核糖体亚单位上引起密码读错，阻断肽酰-tRNA（peptidyl-tRNA）从受体位点到供体点点的转移。 分子式N/A 分子量N/A 储存条件2-8 C 冷藏保存。 50 g/mL in H2O ( is the recommended working concentration for eukaryotic cell culture) Used as a selection agent (gentamicin-resistance gene) in molecular biology applications. Biochem/physiol Actions Mode of action: Gentamicin causes codon misreading by binding to the 30S ribosomal subunit, blocking the translocation of peptidyl-tRNA from the acceptor site to the donor site.1,2 The bactericidal effect of gentamicin on Pseudomonas aeruginosa is exerted by the binding of gentamicin to the outer membrane, where it displaces natural cations, destabilizes the membrane, and forms holes in the cell surface.3 Antimicrobial spectrum: Gram-negative bacteria, Staphylococcus aureus and other Gram-positive bacteria. 参考文献

1. Korzybski, T., et al. Antibiotics: origin, nature, and properties Washington, DC , (1977), 712-723

2. Lorian, V. (ed.) Antibiotics in Laboratory Medicine 2nd ed., Baltimore, MD , (1986), 694-696 3. Kadurugamuwa, J., et al., Surface action of gentamicin on Pseudomonas aeruginosa J. Bacteriol. 175, 5798-5805, (1993) Merck 14,4392