Signosis/Human Apoptosis cDNA Plate Array I/AP-0121/1 Ea-蚂蚁淘商城

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Signosis/Human Apoptosis cDNA Plate Array I/AP-0121/1 Ea

2025-11-13

Description:

Apoptosis is a cellular process involving cytoplasmic condensation and cell shrinkage, chromatin condensation, membrane blebbing, nuclear fragmentation, and the formation of apoptotic bodies, which leads to programmed cell death. During the process, a series of biochemical reactions occur, such as the activation of intracellular proteases and regulation of apoptosis-associated genes. Signosis’ Apoptosis-Associated cDNA Plate Array provides an approach for profiling the expression of apoptosis-regulating genes.

Applicable Grid:

List of Applicable Genes

123456789101112AAIFBIDGAPDHAIFBIDGAPDHAIFBIDGAPDHAIFBIDGAPDHBAPAF1CASP3LTAAPAF1CASP3LTAAPAF1CASP3LTAAPAF1CASP3LTACBADCASP8MCL1BADCASP8MCL1BADCASP8MCL1BADCASP8MCL1DBAKCASP9p53BAKCASP9p53BAKCASP9p53BAKCASP9p53EBAXCYCSSMACBAXCYCSSMACBAXCYCSSMACBAXCYCSSMACFBCL2FASSurvivinBCL2FASSurvivinBCL2FASSurvivinBCL2FASSurvivinGBcl-xLFASLTNFaBcl-xLFASLTNFaBcl-xLFASLTNFaBcl-xLFASLTNFaHBeta-actinFLIPBlankBeta-actinFLIPBlankBeta-actinFLIPBlankBeta-actinFLIPBlank

Principle:

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity. $\"\"$

Data:

$\"\"$ Apoptosis cDNA array analysis of gene expression in HeLa. HeLa cell were treated with and without 2uM camptothcin for 16 hours. Total RNAs were prepared for the cDNA plate array assay.