Mabtech/Bovine IgG ELISpot BASIC (HRP)/3150-2H/for 4 plates
Bovine IgG ELISpotBASIC enables enumeration of B cells secreting total IgG as well as antigen-specific IgG. It contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP and a polyclonal activator.
Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 (included).
IgG ELISpotBASIC is also available with Streptavidin-ALP. See Related products.
As complementary products, we offer ELISpot plates and substrates. See Related products.
Analyte Information
IgGIgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with highest serum concentrations of IgG1 followed by IgG2, IgG3 and IgG4. In mice the IgG subclasses are defined IgG1, IgG2a/c, IgG2b and IgG3. The IgG molecule consists of two heavy and two lights chains (κ or λ) resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
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| for 4 plates |
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| I have compared Mabtech ELISpot assay to ELISpot assays we run at our company on regular basis \"head-to-head\" using identical samples. Mabtechs kits proved to be superior to our protocol, had significantly lower background and provided better and higher detection of spots. |
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