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苏州蚂蚁淘生物科技有限公司成立于2009年,是一家专业提供实验室整体采购方案的专业公司,为广大采购和实验室解决了大量问题,几年来已经成为几乎所有合作伙伴最优秀的合作伙伴,我们一直秉承信誉第一的原则,坚持不卖假货,哪怕利润损失也要为客户提供最好的产品。

我们已经和国外500多个品牌建立了贸易往来,并在国际上享有相当知名度,现已经成为国外近100个品牌中国指定代理,并成为其中国最优秀的代理商。

我们部分客户

1:经销商代表---国药化试,飞世尔,SIGMA,等1000家左右的经销商

2:学校代表---北京大学,清华大学,上海交通大学,复旦大学,浙江大学,武汉大学,厦门大学.....等200所左右大学及100多家附属医院

3:制药公司代表:罗氏,阿斯利康,诺和诺德,和记黄埔,东阳光科......

4:CRO公司代表:睿智化学,药明康德,桑迪亚,美迪西......

我们现有代理品牌如下,还在不断增加中



性能参数

产品名称: 卓越性能,Mobitec巨大芽孢杆菌表达系统
英文名称: Bacillus megaterium Expression Systems
产品型号: kit
产品规格: kit
产品产地: 德国
品牌商标: MOBITEC
价    格: 面议元
 

产品详细描述

 

说明书(bmeg_handbook.pdf)

Features:

  • Stable, high-yield protein expression in Bacillus megaterium 
     
  • Ideal for both small and large-scale protein production
  • Tightly regulated and efficiently inducible xylose operon (up to 350-fold)
  • No alkaline proteases activity even up to 5 hours after induction
  • No endotoxins observed
  • Versatile cloning (extended polylinker, additional BsrG I site*)
  • Versatile production (intracellular or extracellular)
  • Versatile purification (native, 6xHis-tag, Strep-tag, Strep/6xHis double-tag)
  • Removeable tag versions (for TEV or Xa proteases) available New!
  • Compatible with all B. subtilis vectors

*BsrG I site is not included in all vectors.

The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small and large scale.

B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

High Protein Yield
As a result, protein yields are exceptionally high even if inexpensive substrates are used. For example, the proteins mutarotase (Mro) and glucose dehydrogenase (Gdh) have been accumulated up to 20% and 30% of the total soluble protein, respectively. Using the tightly regulated xylose operon, the genes were induced 130- to 350-fold without proteolysis.


Versatile System with a Wide Range of Vectors
MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable for most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence, a 6xHis-tag, a Strep-tag, and a Strep/6xHis double-tag. Additionally, all vectors contain the tightly regulated and highly inducible xylose operon promoter.

B. megaterium protoplasts specifically optimized for transformation 
The protoplasts supplied by MoBiTec are from B. megaterium strain WH320 developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies. 

For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

Examples:

Selected Proteins successfully over-produced in Bacilli strains with our B. megaterium vectors:

  • β-Galactosidase (LacZ)1
  • Catabolite control protein (CcpA)2,3
  • Clostridium difficile toxin A4
  • Cobaltochelatase (CbiX)5
  • Dextransucrase6
  • Endolevanase (LevB)7
  • Glucose dehydrogenase (GdhA)1
  • Heat shock protein (HPr) from PTS (phosphotransferase sugar transport system)8
  • Human single-chain urokinase-like plasminogen activator (rscuPA)1
  • Levansucrase9
  • Mutarotase (Mro)1
  • Neopullulanase10
  • Translocation ATPase of the preprotein translocase (SecA)11
  • Trehalose repressor (TreR)12
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