请使用支持JavaScript的浏览器! 理论探讨:MoBiTec美元价/进货价 德国mobitec,有谁知道吗?-Mo Bi Tec代理咨询-蚂蚁淘商城
当前位置:蚂蚁淘商城 > 品牌馆 > Mo Bi Tec > 品牌咨询 > 理论探讨:MoBiTec美元价/进货价 德国mobitec,有谁知道吗?
品牌咨询

苏州蚂蚁淘生物科技有限公司是一家依托于中科院生命科学领域的高新技术生物公司,致力于生命科学领域产品的研发与销售,蚂蚁淘生物秉承为客户提供高质量产品及优质服务的理念,从事科研机构及生产企业所需的各种品牌生化剂、抗体、血清、ELISA试剂盒、移液枪、中小型仪器设备、耗材等专业进出口公司。主要经营品牌abcam、abnova、peprotech、RD、CST、AbD Serotec、Athens Research Technology、Chemicon 、Evrogen、 Bio-Rad、 Zymed、wako、bachem、Exiqon、Invitrogen、Eppendorf、Bioassay、cayman、TRC、Omega、Promega、Axygen、AssayPro等品牌中国区域.代理商,自成立以来发展迅速,产品服务得到全国广大生产企业、高校、科研院所、医疗系统及相关试剂公司的肯定,我们愿竭尽所能,赛因百奥生物在保证产品质量的基础上将以超低价格为您提供优质服务;同时也为广大经销公司提供充足货源,我们的目标是努力成为国内生物学产品行业专业供应商。


性能参数

产品名称: 卓越性能,Mobitec巨大芽孢杆菌表达系统
英文名称: Bacillus megaterium Expression Systems
产品型号: kit
产品规格: kit
产品产地: 德国
品牌商标: MOBITEC
价    格: 面议元
 

产品详细描述

 

说明书(bmeg_handbook.pdf)

Features:

  • Stable, high-yield protein expression in Bacillus megaterium 
     
  • Ideal for both small and large-scale protein production
  • Tightly regulated and efficiently inducible xylose operon (up to 350-fold)
  • No alkaline proteases activity even up to 5 hours after induction
  • No endotoxins observed
  • Versatile cloning (extended polylinker, additional BsrG I site*)
  • Versatile production (intracellular or extracellular)
  • Versatile purification (native, 6xHis-tag, Strep-tag, Strep/6xHis double-tag)
  • Removeable tag versions (for TEV or Xa proteases) available New!
  • Compatible with all B. subtilis vectors

*BsrG I site is not included in all vectors.

The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small and large scale.

B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

High Protein Yield
As a result, protein yields are exceptionally high even if inexpensive substrates are used. For example, the proteins mutarotase (Mro) and glucose dehydrogenase (Gdh) have been accumulated up to 20% and 30% of the total soluble protein, respectively. Using the tightly regulated xylose operon, the genes were induced 130- to 350-fold without proteolysis.


Versatile System with a Wide Range of Vectors
MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable for most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence, a 6xHis-tag, a Strep-tag, and a Strep/6xHis double-tag. Additionally, all vectors contain the tightly regulated and highly inducible xylose operon promoter.

B. megaterium protoplasts specifically optimized for transformation 
The protoplasts supplied by MoBiTec are from B. megaterium strain WH320 developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies. 

For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

Examples:

Selected Proteins successfully over-produced in Bacilli strains with our B. megaterium vectors:

  • β-Galactosidase (LacZ)1
  • Catabolite control protein (CcpA)2,3
  • Clostridium difficile toxin A4
  • Cobaltochelatase (CbiX)5
  • Dextransucrase6
  • Endolevanase (LevB)7
  • Glucose dehydrogenase (GdhA)1
  • Heat shock protein (HPr) from PTS (phosphotransferase sugar transport system)8
  • Human single-chain urokinase-like plasminogen activator (rscuPA)1
  • Levansucrase9
  • Mutarotase (Mro)1
  • Neopullulanase10
  • Translocation ATPase of the preprotein translocase (SecA)11
  • Trehalose repressor (TreR)12
我要咨询
邮箱:
标题:
咨询:
品牌分类