The one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution.Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.
Benefits of the BioFX® family of substrates include:
- Prolonged stability
- Increased sensitivity
- Negligible backgrounds
- Ease of manufacturing
TTMB
- Product Insert
- Safety Data Sheet (SDS)
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