DRG支付体系构建的国际经验借鉴（DRG定价篇）_新浪医药 ...
来自 : 蚂蚁淘
- LabelingMicrotubules(MolecularDynamicsInc.)Microtubulesareinvolvedinmanyaspectsofcellmotionincludingpropulsion,mitosis,growth,andorganelletransport.Theyarecomposedofa-andß-tubulin,andareabout25nminouterdiameter.Microtubulesareeasilystainedusingananti-tubulinprimaryantibodyandacorrespondingsecondaryantibodyconjugatedtoanyFluorochrome.Theirregularsizeandfinestructureprovidebeautifulimages.Thisprocedurerevealssmoothandlinearmicrotubulesandcanbeusedwithawideusr/localietyofotherantigensaswell.
- AnalysisofIntracellularOrganellesbyFlowCytometryorMicroscopy(CPCProtocols)Functionalanalysisofcellularorganellescanbeaccomplishedbystainingcellswithsuitableorganelle-specificdyesandthenanalyzingthefluorescenceofthestainedcellswithaflowcytometer.WiththismethodologyitispossIBLetoresolvesUSPectedheterogeneityinorganellefunctionorcontentwithinapopulationofcells.Flowcytometrydoesnotprovidemorphologicalinformation;ifthatisdesired,quantitativemicroscopyusingavideomicroscopewithdigitalimageanalysissystem,oraconfocalmicroscopeshouldbeemployed
- Preparationoftubulin(SalmonLab)Simpleprocedurewithhighyielding
- PreparationofRatLiverCellCytosoland/orOrganelleFractions(SalmonLab)
- AdrenalChromaffinGranule(chromaffinvesicle)Preparation(DanielT.O"Connor)Thismethodisusuallyusedtopreparegranulesfromfreshlyobtained(neverfrozen!)bovineadrenalglands,butcanalsobeusedtopreparechromaffingranulesfromfreshhumanpheochromocytoma.
- LysosomeIsolationinIsotonicSucrose(WilliamH.Heidcamp)
- EmObservationsofMicrosomes(WilliamH.Heidcamp)
- LiposomeIsolationfromBacteria(HancockLab)(AccessibleonlybyIE)
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