50 MboI
[NextPage]Enzyme SuppliedNEBuffer %ActivityinNEBuffers 1 2 3 4 AATII 4 0 50 50 100 AccI 4 50 50 10 100 Acc65I 3+BSA 10 75 100 25 AciI 3 25 50 100 50 AclI 4+BSA 10 10 0 100 AfeI* SE-Y 25 50 25 100 AflII 2+BSA 50 100 25 100 AflIII 3+BSA 25 75 100 50 AgeI 1 100 50 10 75 AhdI 4+BSA 25 75 0 100 AleI 4 10 20 10 100 AluI 2 100 100 75 100 AlwI 4 50 100 10 100 AlwNI 4 50 100 50 100 ApaI@25°C 4+BSA 25 50 0 100 ApaLI 4+BSA 100 100 10 100 ApoI@50°C 3+BSA 10 75 100 75 AscI 4 0 10 10 100 AseI 3 NR 75 dd100 NR AsiSI 3+BSA 50 100 100 50 AvaI 4 10 75 10 100 AvaII 4 50 75 10 100 AvrII 2 100 100 50 100 BaeI@25°C 2+BSA+SAM 50 100 50 75 BamHI Udd+BSA 75 100 50 75 BanI 4 50 100 50 100 BanII 4 100 10 50 100 BbsI 2 100 100 25 75 BbvI 2 100 100 25 75 BbvCI 4 50 100 10 100 BceAI Udd+BSA 25 NR NR 75 BcgI U+SAM 50 75 100 50 BciVI 4 100 25 10 100 BclI@50°C 3 50 100 dd100 75 BfaI 4 75 50 10 100 BfrBI 4+BSA 10 25 10 100 BfuAI@50°C 3 0 75 100 10 BfuCI 4+BSA 100 50 25 100 BglI 3 50 75 100 50 BglII 3 10 75 100 10 BlpI 4 50 100 10 100 Bme1580I 2 75 100 75 75 BmgBI 3+BSA 0 25 100 10 BmrI 2 75 100 75 100 BmtI* SE-W 25 100 10 50 BpmI 3+BSA 75 100 100 100 Bpu10I* SE-K 0 25 100 25 BpuEI@25°C 2+SAM 75 100 10 75 BsaI@50°C 3 75 75 100 100 BsaAI 3 75 75 100 75 BsaBI@60°C 2 75 100 100 100 BsaHI 4+BSA 50 100 100 100 BsaJI@60°C 2 100 100 100 100 BsaWI@60°C 2+BSA 50 100 50 50 BsaXI 4 BseRI 2 100 100 75 100 BseYI 3 10 50 100 50 BsgI 4+SAM 50 75 50 100 BsiEI@60°C 2+BSA 50 100 10 100 BsiHKAI@65°C 3+BSA 50 100 100 100 BsiWI@55°C 3 100 100 100 25 BslI@55°C 3 10 50 100 75 BsmI@65°C 2 75 100 75 75 BsmAI@55°C 3 50 100 100 50 BsmBI@55°C 3 75 100 100 100 BsmFI@65°C 4+BSA 25 50 50 100 BsoBI@65°C 2 10 100 100 50 Bsp1286I 4+BSA 25 25 25 100 BspCNI@25°C 1+BSA+SAM 100 75 10 75 BspDI 4 25 75 50 100 BspEI 3 0 10 100 0 BspHI 4 10 100 50 100 BspMI 3 NR NR 100 NR BsrI@65°C 3 0 50 100 10 BsrBI 2 50 100 100 100 BsrDI@60°C 2+BSA 50 100 50 75 BsrFI U 25 100 75 75 BsrGI 2+BSA 25 100 10 100 BssHII@50°C 3 100 100 dd100 100 BssKI@60°C 3+BSA 0 50 100 50 BssSI 3 0 50 dd100 10 BstAPI*@60°C SE-W 25 25 100 25 BstBI@65°C 4 75 50 25 100 BstEII@60°C 3 50 75 100 75 BstF5I*@65°C SE-Y 75 50 25 100 BstNI@60°C 2+BSA 10 100 100 75 BstUI@60°C 2 100 100 50 100 BstXI@55°C 3 25 100 100 50 BstYI@60°C 2 50 100 75 100 BstZ17I 3 NR NR 100 NR Bsu36I 3+BSA 0 25 100 0 BtgI 3+BSA 25 50 100 100 BtsII 4+BSA Cac8I 3 50 75 100 75 ClaI 4+BSA 10 50 50 100 DdeI 3 75 100 100 75 DpnI 4 100 100 75 100 DpnII U NR NR dd100 NR DraI 4 75 75 50 100 DraIII 3+BSA 100 75 100 25 DrdI 4 25 50 10 100 EaeI 1 100 100 50 100 EagI 3 10 25 100 10 EarI 1 100 100 50 100 EciI 2+BSA 50 100 100 50 EcoNI 4 100 100 75 100 EcoO109I 4+BSA 100 100 75 100 EcoRI Udd 100 100 100 100 EcoRV 3+BSA 50 75 100 50 FatI* SE-G 10 100 50 50 FauI*@55°C SE-B 100 25 10 50 Fnu4HI 4 10 25 25 100 FokI 4 50 100 75 100 FseI 4+BSA 100 75 0 100 FspI 4 10 75 10 100 HaeII 4+BSA 75 100 50 100 HaeIII 2 50 100 25 75 HgaI 1 100 75 50 100 HhaI 4+BSA 75 100 100 100 HincII 3+BSA 75 100 100 100 HindIII 2 50 100 10 50 HinfI 2 75 100 75 75 HinP1I 2 100 100 100 100 HpaI 4 25 50 10 100 HpaII 1 100 50 10 100 HphI 4 NR 75 0 100 Hpy99I 4+BSA Hpy188I 4 Hpy188III 4+BSA HpyCH4III 4 HpyCH4IV 1 HpyCH4V 4 I-CeuI 4+BSA 10 10 0 100 I-SceI U+BSA 10 50 50 50 KasI 2+BSA 75 100 75 75 KpnI 1+BSA 100 75 0 3 75 100 100 100 MboII 2 100 100 50 100 McrBC 2+BSA+GTP 50 100 10 75 MfeI 4 75 50 10 100 MluI 3 25 75 100 50 MlyI 4+BSA 50 50 25 100 MmeI 4+SAM NR NR NR 100 MnlI 2+BSA 75 100 50 75 MscI 4 75 75 75 100 MseI 2+BSA 75 100 75 75 MslI 2 100 100 25 100 MspI 2 75 100 50 50 MspA1I 4+BSA 50 100 50 100 MwoI@60°C U 10 75 100 75 NaeI 1 100 75 10 75 N.AlwI 2 25 100 25 50 NarI 1 100 75 75 75 N.BbvCIA 2 50 100 10 100 N.BbvCIB 2 50 100 10 100 N.BstNBI@55°C U 0 10 10 10 NciI 4 100 25 10 100 NcoI 4 100 100 100 100 NdeI 4 75 100 75 100 NgoMIV 4 100 50 10 100 NheI 2+BSA 100 100 10 100 NlaIII 4+BSA 25 25 25 100 NlaIV 4+BSA 10 10 10 100 NotI 3+BSA 0 75 100 50 NruI U 0 10 75 10 NsiI U 25 25 50 10 NspI 2+BSA 100 100 0 100 PacI 1+BSA 100 75 10 100 PaeR7I 4 25 100 10 100 PciI* SE-O 25 75 100 50 PflFI 4+BSA 0 25 25 100 PflMI 3+BSA 0 100 100 50 PI-PspI@65°C U+BSA 0 10 10 10 PI-SceI U+BSA 0 0 0 0 PleI 4 100 100 75 100 PmeI 4+BSA 0 50 10 100 PmlI 1+BSA 100 75 0 75 PpuMI 4 0 25 0 100 PshAI 4+BSA 50 75 10 100 PsiI* SE-B 50 100 0 25 PspGI@75°C 2 75 100 50 100 PspOMI* SE-Y 25 100 50 100 PstI 3+BSA 75 75 100 50 PvuI 3+BSA 10 75 100 10 PvuII 2 100 100 100 100 RsaI 1 100 100 50 100 RsrII 4 25 75 10 100 SacI 1+BSA 100 50 10 100 SacII 4 25 75 10 100 SalI U+BSA 0 0 50 0 SapI 4 75 50 0 100 Sau3AI U+BSA 100 50 10 100 Sau96I 4 50 100 100 100 SbfI* SE-Y 75 50 0 100 ScaI U 25 75 dd50 25 ScrFI 4 100 100 100 100 SexAI 3+BSA 25 25 100 100 SfaNI 3 0 75 100 25 SfcI 4+BSA 75 50 10 100 SfiI@50°C 2+BSA 0 100 10 100 SfoI 2 25 100 50 50 SgrAI 4 100 50 10 100 SmaI@25°C 4 0 0 0 100 SmlI@55°C 4+BSA 25 75 25 100 SnaBI 4+BSA 25 50 25 dd100 SpeI 2+BSA 75 100 25 75 SphI 2 100 100 50 100 SspI U 50 100 50 50 StuI 2 100 100 50 100 StyI 3+BSA 25 75 100 10 StyD4I 2 10 100 100 100 SwaI@25°C 3+BSA 10 10 100 10 TaqI@65°C U+BSA 50 75 75 75 TfiI@65°C 3 100 100 dd100 100 TliI@75°C 3+BSA 25 25 100 10 TseI@65°C 3 75 100 100 75 Tsp45I@65°C 1+BSA 100 25 0 75 Tsp509I@65°C 1 100 100 100 NR TspRI@65°C 4+BSA 25 50 25 100 Tth111I@65°C 1 100 25 25 100 XbaI 2+BSA 0 100 75 75 XcmI 2 10 100 50 50 XhoI 2+BSA 75 100 100 100 XmaI 4+BSA 25 50 0 100 XmnI 2+BSA 100 100 75 100 ZraI* SE-B 100 100 25 100
