LOCUS pEarleyGate 202 11776 bp ds-DNA circular SYN 26-JUL-2016DEFINITION Gateway(R)-compatible plant transformation vector with an N-terminal FLAG tag.SOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 11776) AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS. TITLE Gateway-compatible vectors for plant functional genomics and proteomics. JOURNAL Plant J. 2006;45:616-29. PUBMED 16441352REFERENCE 2 (bases 1 to 11776) AUTHORS Pikaard Lab TITLE Direct Submission JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector NTI(R) format http://www.snapgene.comCOMMENT The sequence was corrected by inserting a G at position 7883.COMMENT VNTDATE|660009600|COMMENT VNTDBDATE|776390400|COMMENT VNTNAME|pEarleyGate 202|COMMENT VNTAUTHORNAME|Pikaard Lab|FEATURES Location/Qualifiers misc_feature 1..25 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 111..363 /label=MAS terminator /note="mannopine synthase terminator" CDS complement(373..924) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase草丁膦（磷丝菌素） 乙酰转移酶" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" promoter complement(930..1310) /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" promoter 2306..2651 /label=35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" misc_feature 2681..2735 /label=TMV Omega /note="translational enhancer from the tobacco mosaic virus 5-leader sequence (Gallie et al., 1988)" CDS 2737..2739 /codon_start=1 /product="start codon" /label=ATG CDS 2740..2763 /codon_start=1 /product="FLAG epitope tag, followed by an enterokinase cleavage site" /label=FLAG protein_bind 2767..2886 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 2916..2946 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 3000..3659 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" CDS 4001..4306 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." protein_bind complement(4347..4471) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" terminator 4547..5254 /label=OCS terminator /note="octopine synthase terminator" misc_feature 5498..5522 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 6822..7451 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label=pVS1 StaA CDS 7880..8953 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label=pVS1 RepA /translation="MDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 9019..9213 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 9557..9697 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(9883..10471) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10558..11352) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin"ORIGIN 1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg 61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat 121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat 181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa 241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc 301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc 361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct 421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg 481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg 541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg 601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga 661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc 721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc 781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat 841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc 901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat 961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt 1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc 1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt 1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga 1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa 1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat 1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg 1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata 1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa 1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac 1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa 1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc 1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt 1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac 1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt 1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga 1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat 1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat 2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt 2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac 2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga 2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata 2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc 2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag 2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg 2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg 2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg 2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt 2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa 2701 acaacaaaca acattacaat tacatttaca attaccatgg actacaaaga cgatgacgac 2761 aaaatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat 2821 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc 2881 cagtcatatt ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt 2941 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa 3001 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac 3061 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata 3121 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc 3181 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg 3241 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa 3301 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt 3361 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga 3421 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg 3481 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg 3541 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg 3601 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa 3661 cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt 3721 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat 3781 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat 3841 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt 3901 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa 3961 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac 4021 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac 4081 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc 4141 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga 4201 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga