【教学参考】基因治疗:基因类疾病治愈的希望_腾讯新闻
| 货号： | TC20 |
| 保修期： | 一年 |
| 现货状态： | 有 |
| 供应商： | 北京赛百奥 |
TheTC20automatedcellcountercountsmammaliancellsinonesimplestepusingitsinnovativeauto-focustechnologyandsophisticatedcellcountingalgorithmtoproduce accuratecellcountsinlessthan30seconds.Uponinsertionofacountingslide,theTC20cellcounterrapidlyprovidesatotalcellcount(withorwithouttrypanbluestaining)andassessescellviabilityviatrypanblueexclusion.
KeyFeaturesandBenefits
· Compatiblewithabroadrangeofcellsizesandtypes —countscelllines,primarycells(fromtissueorblood),andstemcells
· Innovativeauto-focustechnology —removesthevariationassociatedwithmanualfocusingandleadstoprecisecellcountsin30seconds
· Cellsizegates —userselectsapopulationofinterestincomplexsamples,suchasprimarycells,orletsthecellcountingalgorithmdoallthework
· Cellviability —analyzescellsaccuratelyusing multifocalplaneanalysis
· Easytoarchiveandanalyze —storesupto100countsintheonboardmemoryforaccessanytime,orusetheoptional TC20dataanalyzersoftwareonyourPCtofurtheranalyzeexportedcellimages
Gettingallthedatayouneedaboutyourcellculturesisfastandeasy;theTC20cellcounteranddisposablecountingslideseliminatetheneedfortedioussetup,cleaning,ormaintenance.TheTC20cellcounterissimpleandintuitivetouse
GetAccurateandReproducibleResults
TheTC20automatedcellcounterusesmicroscopywithauto-focusthatanalyzesmultiplefocalplanestoidentifythebestplane.Withoutrequiringanyuserinput,thesophisticatedcellcountingalgorithmusestheimageacquiredfromthebestfocalplanetoidentifycellsandexcludedebris,therebycalculatingthetotalcellcount.Theauto-focusleadstohighlyreproduciblecellcountswithreduceduser-to-uservariabilitycomparedtoahemocytometerandcellcounterswithmanualfocus.Usingauto-focusinsteadofsubjectivemanualfocusingisespeciallyimportantwhenassessingcellviabilitybecauseanincorrectlyselectedfocalplanewillleadtoinaccurateresults.
Unlikecellcountersbasedonelectricalimpedance(Coulterprinciple),theTC20image-basedanalysisallowstheusertoviewthecellsinrealtime,providingvisualproofthattheTC20cellcounterhascorrectlyidentifiedcells.
TheTC20automatedcellcounterprovidesaccuracycomparabletoresultsobtainedwithahemocytometer.Itcancountcellswitha6–50μmcelldiameterandwithinabroadconcentrationrangeof5x104–1x107cells/ml,whicheliminatestheneedtodilutecells,thusreducingerrorsassociatedwithsampledilutionspriortocounting.
Thecounterusesdisposablecountingslideswithapatent-pendingdesignthatensuresevendistributionofcellsthroughoutthecountingchamber,regardlessoftheuserspipettingstyle,leadingtoaccurateandconsistentcellcounts.
Forcomplexsamplescomposedofmultiplecellpopulations,suchasprimarycells,userscanadjustthecellsizegatestodefinethepopulationofinterestthatwillbecounted.Whencountingmultiplesamplereplicates,theTC20cellcountercansavethepositionofthecellsizegatesandapplythemtosubsequentcounts.
CellViability
TheTC20cellcounteruses multifocalplaneanalysistoassesscellviability.Theconventionalmethodofanalyzingviabilityusingasinglefocalplanecanleadtoinaccurateconclusionsbecauselightscatteringandthealignmentofcellsatdifferentheightsinacountingchambercanchangetheappearanceofcells—livecellsmayappeartobedeadandviceversa.Todetermineifcellsareviable,theTC20counteranalyzeseachcellusingimagesacquiredfrommultiplefocalplanesduringthefocusingstep.
MinimizeYourSamplePreparation
ThebroadconcentrationrangeoftheTC20cellcountereliminatestheneedtodilutecellspriortocounting,reducingtheerrorsassociatedwithsampledilutionsthatmaybenecessarywhencountingcellsbyothermethods.Thecountingalgorithmdiscriminatesandcountsindividualcellswithinclustersofuptofivecells,providingaccuratecountswithouttheneedtoextensivelydeclumpcellspriortoloading.
Countingtime,sec | 30 |
Cellconcentrationrange,cells/ml | 5x104–1x107 |
Optimalcellconcentrationrange,cells/ml | 1x105–5x106 |
Celldiameterrange,µm | 6–50 |
Samplevolume,µl | 10 |
Datastorage,counts | 100 |
Dataexport | ViaUSBflashdrive |
Printerconnectivity | Yes |
Dilutioncalculator | Yes |
Imageofcellsavailable | Yes |
Dimensions(WxDxH),cm(in) | 19x15x25.4(7.5x6x10) |
Weightwithouttheexternalpowersupply,kg(lb) | 2.2(4.8) |
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发布于 ： 2019-11-03
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