
- SpeciesReactivityMouse
- SpecificityDetectsmouseSLAM/CD150.
- SourceMonoclonalRatIgG2AClone#459911
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenMousemyelomacelllineNS0-derivedrecombinantmouseSLAM/CD150
Thr25-Pro242
Accession#Q9QUM4 - FormulationSuppliedinasalinesolutioncontainingBSAandSodiumAzide.
- LabelFluorescein
- FlowCytometry10µL/106cellsSeebelow
- ShippingTheproductisshippedwithpolarpacks.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.
- StABIlity&StorageProtectfromlight.Donotfreeze.
- 12monthsfromdateofreceipt,2to8°Cassupplied.
- Ma,C.S.etal.(2007)Annu.Rev.Immunol.25:337.
- Castro,A.G.etal.(1999)J.Immunol.163:5860.
- Cocks,B.G.etal.(1995)Nature376:260.
- Wang,N.etal.(2004)J.Exp.Med.199:1255.
- Hahm,B.etal.(2004)Virology323:292.
- Nanda,N.etal.(2005)Blood106:3028.
- Kiel,M.J.etal.(2005)Cell121:1109.
- Punnonen,J.etal.(1997)J.Exp.Med.185:993.
- Mavaddat,N.etal.(2000)J.Biol.Chem.275:28100.
- Aversa,G.etal.(1997)J.Immunol.158:4036.
- Mehrle,S.etal.(2008)Mol.Immunol.45:796.
- Wang,N.etal.(2006)Am.J.Respir.CellMol.Biol.35:206.
- Tatsuo,H.etal.(2000)Nature406:893.
- Hsu,E.C.etal.(2001)Virology279:9.
- LongName:SignalingLymphocyticActivationMolecule
- EntrezGeneIDs:6504(Human);27218(Mouse)
- AlternateNames:CD150;CD150;CD150IPO-3;CDw150;IPO-3;signalinglymphocyticactivationmoleculefamilymember1;signalinglymphocyticactivationmolecule;SLAM;SLAMCD150antigen;SLAMF1
Background:
ThetypeItransmembraneglycoproteinSignalingLymphocyticActivationMolecule(SLAM),alsoknownasCD150,istheprototypicmemberoftheSLAMsubgroupoftheCD2proteinfamily.CD2familyproteinsfunctionasadhesionmoleculesandmodulatorsoftheimmuneresponse(1).MouseSLAMconsistsofa218aminoacid(aa)extracellulardomain(ECD)withtwoIg-likedomains,a23 aatransmembranesegment,anda78aacytoplasmicdomainwiththreeimmunoreceptortyrosineswitchmotifs(ITSM)(2).Alternatesplicinggeneratesanisoformwithasubstitutedcytoplasmicdomain(2).WithintheECD,mouseSLAMshares58%and83%aasequenceidentitywithhumanandratSLAM,respectively.Itisexpressedasa75kDamoleculeofwhichapproximately30kDaisN-linkedcarbohydrate(2).SLAMisexpressedonTcells,Bcells,thymocytes,macrophages,dendriticcells,platelets,andhematopoieticstemcells(2‑7).ItisupregulatedonactivatedBcellsandCD4+andCD8+Tcells,althoughitisdownregulatedonTh2polarizedcells(2, 3, 8).SLAMinteractshomophilicallywithlowaffinity,andthisinteractioninducesaTh0/Th1responsecharacterizedbyclonalexpansion,productionofIFN-gamma,andincreasedcytolyticactivityofCD8+Tcells(2, 3, 9‑11).SLAMligationalsopromotesBcellactivation,allergen-inducedeosinophilandmastcellactivation,andmacrophageresponsivenesstoLPS(4, 8, 12).Inhumans,SLAMfunctionsasacellularentryreceptorformeaslesvirus(13, 14).
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此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
500ml
2。阴极缓冲液 ( 10 × ) :将 60.55g Tris ,89.58g Tricine 及 5g SDS 溶于400ml 蒸馏水中,加水至终体积为500ml
使用时稀释至1×的缓冲液,电泳槽内槽加入阴极电泳缓冲液,外槽加入阳极电泳缓冲液。形成Trcine-SDS-PAGE电泳系统。


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