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| (±)-Bay K 8644L-type Ca2+-channel activator |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.
Science.2016 Aug 5;353(6299)594-8Nat Nanotechnol.2017 Dec;12(12):1190-1198.Nature Biotechnology.2017 Jun;35(6):569-576Nat Med.2018 Sep 17.Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.Cell.Available online 25 October 2018.Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.Cell.2018 Jun 28;174(1):172-186.e21.Cell.2018 Feb 22;172(5):1007-1021.e17.Cell.2017 Nov 30;171(6):1284-1300.e21.Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.Cell.2017 Jul 13;170(2):312-323Nat Med.2018 Jan 29.Nat Med.2017 Nov;23(11):1342-1351.Cell.2017 Apr 6;169(2):286-300.Cell.2015 Aug 27;162(5):987-1002.Cell.2015 Feb 12;160(4):729-44.Nature Medicine.2017 Apr;23(4):493-500.Cancer Cell.2018 May 14;33(5):905-921.e5.Cancer Cell.2018 Apr 9;33(4):752-769.e8.Cancer Cell.2018 Mar 12;33(3):401-416.e8.Cancer Cell.2017 Aug 14;32(2):253-267.e5.Nat Methods.2018 Jul;15(7):523-526.Cell Stem Cell.2018 May 3;22(5):769-778.e4.Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.| Cell experiment [1]: | |
Cell lines | Guinea Pig and Calf Myocardial Cells |
Preparation method | Soluble to 100 mM in ethanol. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reacting condition | 280 nM, 5 minutes |
Applications | Bay k 8644 increased twitch tension in guinea pig atria without changing the time course of tension development. Bay k 8644 increased the action potential duration of calf ventricular muscle and Purkinje fibers. Bay k 8644 increased strontium currents and altered the time- and voltage-dependence of channel opening. |
| Animal experiment [2]: | |
Animal models | Male Sprague-Dawley rats |
Dosage form | Intraperitoneal administration, 0.5-4 mg/kg |
Application | Intraperitoneal administration of BAY K 8644 (0.5-4 mg/kg) induced an increase in blood pressure associated with bradycardia, increased tail-flick latency in response to radiant heat, decreased locomotion, induced muscle contraction, postural changes and also reduced reflex activity. BAY K 8644 (4 mg/kg, i.p.) significantly increased homovanillic acid and 3,4-dihydroxyphenylacetic acid concentrations in the cortex and striatum. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Thomas G, Chung M, Cohen C J. A dihydropyridine (Bay k 8644) that enhances calcium currents in guinea pig and calf myocardial cells. A new type of positive inotropic agent[J]. Circulation research, 1985, 56(1): 87-96. [2]. Bourson A, Moser P C, Gower A J, et al. Central and peripheral effects of the dihydropyridine calcium channel activator BAY K 8644 in the rat[J]. European journal of pharmacology, 1989, 160(3): 339-347. | |
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| Cas No. | 71145-03-4 | SDF | Download SDF |
| Chemical Name | methyl 2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl)phenyl]-1,4-dihydropyridine-3-carboxylate | ||
| Canonical SMILES | CC1=C(C(C(=C(N1)C)[N+](=O)[O-])C2=CC=CC=C2C(F)(F)F)C(=O)OC | ||
| Formula | C16H15F3N2O4 | M.Wt | 356.3 |
| Solubility | Soluble in ethanol | Storage | Store at -20°C |
| Physical Appearance | Yellow solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
EC50: Acting as a L-type Ca2+ channel activator with EC50 of 17.3 nM .
The advent of calcium channel activators makes it possible to increase the amount of ACh released from the nerve terminals during their activation. Being applied as a Ca2+ channel activator, BAY K 8644 generally exhibits positive inotropic and vasoconstrictor effects on heart and smooth muscle. [1]
In vitro: It was demonstrated that Bay K 8644 prolonged the mean Ca2+ channel opening time in heart myocytes and neurones of spinal ganglia. An experiment using rat heart ventricles demonstrated that Bay K 8644, at the final concentration of approximately 1 pM, had strong positive inotropic effect when added to the perfusion fluid. Moreover, the addition of Bay K 8644 to the chronic ethanol treatment significantly reduced the electrophysiological signs of withdrawal in the isolated hippocampal slices. [2, 3]
In vivo: Study in mice demonstrated that Bay K 8644 significantly ameliorated the ethanol withdrawal syndrome. When experimental animals were administered with an acute injection of Bay K 8644, the convulsive behavior of mice could be monitored to increase for 2 hours. In addition, BAY k 8644 was also reported to ameliorate hypotension in endotoxin-shocked rats. It could lead to a 37% decrease in heart rate of endotoxin-treated rats and 39% decrease in control rats in a dose-dependent manner. [3,4]
Clinical trial: So far, no clinical trial has been conducted.
References:[1]Greenberg DA, Cooper EC and Carpenter C. Calcium channel "agonist" BAY K 8644 inhibits calcium antagonist binding to brain and PC12 cell membranes. Brain Res. 1987. 305: 3658. [2]Doledal V and Tucek S. Failure of the calcium channel activator, Bay K 8644, to increase the release of acetylcholine from nerve terminals in brain and diaphragm. Br. J. Pharmac. 1987. 91: 475-9.[3]Whittington MA, Butterworth AR, Dolin SJ, Patch TL and Little HJ. The effects of chronic treatment with the dihydropyridine, Bay K 8644, on hyperexcitability due to ethanol withdrawal, in vivo and in vitro. Br. J. Pharmacol. 1992. 105: 285-92.[4] Ives N, King JW, Chernow B and Roth BL. BAY k 8644, a calcium channel agonist, reverses hypotension in endotoxin-shocked rats. Eur J Pharmacol. 1986. 130: 169-175.
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我想用共聚焦观察间期染色体，用涂染探针，有一个问题很困扰我，我想涂染完染色后用DAPI复染细胞核，但是目前哈尔滨的共聚焦都没有紫外激发光，不能激发DAPI，我怎么才能实现，用红色涂一条染色体，用绿色涂另一条，用DAPI蓝色染核，同时成像呢，所说的双光子显微镜可以么？我实在很迷惑，求求版主别再删我的帖子，尽管我现在只是一个索取者，还不能给大家提供有用的信息，但是相信有一天会为大家做贡献的，谢谢了
