RecombinantantibodiesencodedbyIGHV1-69reactwithpUL32,aphosphoproteinofcytomegalovirusandB-cellsuperantigen.
Blood2012Mar8;119(10):2293-301PMID:22234695查看当期期刊
SteiningerC,WidhopfGF2nd,GhiaEM,MorelloCS,VanuraK,SandersR,SpectorD,GuineyD,JgerU,KippsTJ
DepartmentofMedicineI,MedicalUniversityofVienna,Austria.
主要主题词：
Antibodies,Monoclonal;B-Lymphocytes;Cytomegalovirus;ImmunoglobulinHeavyChains;Phosphoproteins;Superantigens
Leukemiacellsfrompatientswithchroniclymphocyticleukemia(CLL)expressahighlyrestrictedimmunoglobulinheavyvariablechain(IGHV)repertoire,suggestingthatalimitedsetofantigensreactswithleukemiccells.Here,weevaluatedthereactivityofapanelofdifferentCLLrecombinantantibodies(rAbs)encodedbythemostcommonlyexpressedIGHVgeneswithapanelofselectedviralandbacterialpathogens.SixdifferentCLLrAbsencodedbyIGHV1-69orIGHV3-21,butnotaCLLrAbencodedbyIGHV4-39genes,reactedwithasingleproteinofhumancytomegalovirus(CMV).TheCMVproteinwasidentifiedasthelargestructuralphosphoproteinpUL32.Incontrast,noneoftheCLLrAbsboundtoanyotherstructureofCMV,adenovirusserotype2,SalmonellaentericaserovarTyphimurium,orofcellsusedforpropagationofthesemicroorganisms.MonoclonalantibodiesorhumanizedrAbsofirrelevantspecificitytopUL32didnotreactwithanyoftheproteinspresentinthedifferentlysates.Still,rAbsencodedbyagermlineIGHV1-6951p1allelefromCMV-seropositiveand-negativeadultsalsoreactedwithpUL32.TheobservedreactivityofmultipledifferentCLLrAbsandnaturalantibodiesfromCMV-seronegativeadultswithpUL32isconsistentwiththepropertiesofasuperantigen.
