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气浴恒温摇床特点及用途
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DescriptionThetubulinfamilyincludesfivedistincttubulins;alpha,beta,gamma,deltaandepsilon.Gamma-tubulinhasaroleinthenucleationandpolarorientationofmicrotubulesandisfoundatthemicrotubuleorganizingcenterssuchasthespindlepolesorthecentrosome.Theantigensequenceisfoundinbothhumangamma-tubulin1andgamma-tubulin2.
BatchNo.Seeproductlabel
Unitsize100µg
AntigenSyntheticpeptide(EEFATEGTDRKDVFFY)conjugatedtoKLHcorrespondingtotheregionofaminoacids38-53ofhumangamma-Tubulin.
CloneIMD-20
OtherNamesTubulingamma-1chain;Gamma-1-tubulin;Gamma-tubulincomplexcomponent1;GCP-1;TUBG1;TUBG;Tubulingamma-2chain;Gamma-2-tubulin;TUBG2;
AccessionP23258TBG1_HUMAN;Q9NRH3TBG2_HUMAN;
ProducedinMouse
PurityIgG
ApplicationsWesternBlotting(WB).Aconcentrationof0.25-0.5µg/mlisrecommendedforWB.HumanTubulingamma-1chainhasapredictedlengthof451residuesandMWof51kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen.
Cross-reactivityHuman;rat;
BlastitIfyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample.
FormLyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3
ReconstitutionReconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial.
StorageAtleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase
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