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以循环方式,移动序列的最佳实践_scala_酷徒编程知识库
来自 : mayitao
货号:AE62701RB
样本:Serum,Plasma,OtherBIOLOGicalfluids
适应物种:Rabbit(Oryctolaguscuniculus)
应用:Qualitative
检测方法:Sandwich
检测限:RequestInformation
供应商:华夏远洋
数量:999
规格:96T/48T
简写:IgG
别名:N/A
检测范围:RequestInformation
产品概述:
特异性:ThisassayhashighsensitivityandexcellentspecificityfordetectionofRabbitIgG.Nosignificantcross-reactivityorinterferencebetweenRabbitIgGandanalogueswasobserved.
回收率:MatriceslistedbelowwerespikedwithcertainlevelofrecombinantRabbitIgGandtherecoveryrateswerecalculatedbycomparingthemeasuredvaluetotheexpectedamountofRabbitIgGinsamples.
线性:ThelinearityofthekitwasassayedbytestingsamplesspikedwithappropriateconcentrationofRabbitIgGandtheirserialdilutions.Theresultsweredemonstratedbythepercentageofcalculatedconcentrationtotheexpected.
稳定性:ThestABIlityofELISAkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Thelossratewasdeterminedbyacceleratedthermaldegradationtest.Keepthekitat37°Cfor4and7days,andcompareO.D.valuesofthekitkeptat37°Cwiththatofatrecommendedtemperature.(referringfromChinaBiologicalProductsStandard,whichwascalculatedbytheArrheniusequation.ForELISAkit,4daysstorageat37°Ccanbeconsideredas6monthsat2-8°C,whichmeans7daysat37°Cequaling12monthsat2-8°C).
测试原理:Thisassayemploysatwo-sitesandwichELISAtoquantitateIgGinsamples.AnantibodyspecificforIgGhasbeenpre-coatedontoamicroplate.StandardsandsamplesarePipettedintothewellsandanyIgGpresentisboundbytheimmobilizedantibody.Afterremovinganyunboundsubstances,abiotin-conjugatedantibodyspecificforIgGisaddedtothewells.Afterwashing,StreptavidinconjugatedHorserADIshPeroxidase(HRP)isaddedtothewells.Followingawashtoremoveanyunboundavidin-enzymereagent,asubstratesolutionisaddedtothewellsandcolordevelopsinproportiontotheamountofIgGboundintheinitialstep.Thecolordevelopmentisstoppedandtheintensityofthecolorismeasured.
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