Albumin Depletion Plus Low Abundance Serum Protein Enrichment With Optimized On-Bead Digestion for LC-MS Label and Label-free Analyses
- Albumin and transferrin voids in flow-through >95%, with <30 minute bind/wash microfuge protocol
- Low abundance enrichment equivalent or better than hexa-peptides or immuno-affinity
- Consumable, cost-effective, no column regeneration or cross-contamination
- Species agnostic; human, rat, mouse, goat, sheep, porcine and bovine sera have been tested
- Trypsin digestion on the bead
- Seamless workflows and unique proteolytic efficiencies
- No in-gel digests, no solution digests, no C18 desalting, more consistent, reproducible results
- Compatibility with quantitative label (i.e., iTRAQ) and label-free LC-MS methods
AlbuVoid™ LC-MS On-Bead is an albumin depletion reagent kit. It removes albumin from serum and plasma samples while concentrating low abundance proteins on the beads. Consequently, the low abundance serum proteins are enriched. It is ideal for applications involving LC-MS discovery and targeted proteomics.
The AlbuVoid™ beads are derived from a silica-based library of individual mixed-mode polymeric ligands. The library was designed to facilitate weak binding of proteins, allowing for progressive enrichment of the low abundance proteome, with specialized voiding properties empirically derived.
The total AlbuVoid™ LC-MS On-Bead proteins were compared for human and rat sera at two different digestion times, 4 hours and overnight (O/N). Note that many identified proteins overlap while certain populations of proteins were only observed in one or the other digest time. Download the detailed report
The AlbuVoid™ LC-MS On-Bead product and protocol is compatible with both label and label-free quantification of peptides/proteins. In the example below, iTRAQ labeled peptides from two representative proteins observed to be differentially quantified in comparing pooled sera from normal and cancer patients.
Click Here For The AlbuVoid™ LC-MS On-Bead For Serum Proteomics Product Sheet美国BSG(BiotechSupportGroup""LLC)公司是一家公司是一家全球知名的蛋白质组和基因组样本预处理的公司,是这个领域公认的领导者,该公司将其发明的专利表面化学技术应用生物学领域,比其他方法如亲合技术,苯酚法有绝对的优势。由于技术上的领先,使得BSG公司的产品广泛被美国国家基因组研究所,斯坦福大学等重要机构使用。
美国BSG(Biotech Support Group,LLC)公司是一家公司是一家全球知名的蛋白质组和基因组样本预处理的公司,是这个领域公认的*,该公司将其发明的表面化学技术应用生物学领域,比其他方法如亲合技术,苯酚法有绝对的优势。由于技术上的,使得BSG公司的产品广泛被美国国家基因组研究所,斯坦福大学等重要机构使用。(http://www.biotechsupportgroup。。com/) 目前我司成功取得美国BSG中国总代理,BSG各大产品均可在我司查询订购。 分子生物学 Ambion,Biosearch Technologies,Biotium,BPS bioscience,Dharmacon,Glen Research,HYQ ,Lucigen, lumiprobe,MO BIO,Molecular probes,NEB,Qiagen 细胞生物学 AddEXBIO,Cellgro,Echelon,Equitech,Gemini,Gibco,InvivoGen,Mlltenyi,Partec,Spherotech,Stemcell Abbiotec,Abcam,ABD SEROtec,Abnova,ADI,Antibodies-Online,AssayPro,Biorbyt,BioVision,BTI,Cellgro,CST,chromotek,Covance,Echelon,Endocrine Tech,Epitomics ,FD Neuro Technologies,Fitzgerald,GeneTex,GenWay,Gibco,Invitrogen,Invivogen,Jackson,Lifespan,LKT Labs,Miltenyi,Novex,Novus,Phoenix peptide,Pierece,,Plantmedia,Prospec,R&D,Spherotech,Stemcell,Trevigen,Santa cruz,,Separopore,USBIo,Vector Beckman coulter,BD,Biolegend,Affymetrix eBioscience,Tonbo A.G.Scientific,American Peptide,Apollo scientific,Cargille Lbs,Cayman,Nanocs,polysciences,Sigma,Tocris 诊断 Biocare,Biospacific,DIARECT,Fujirebio Diagnostics,Roche,Kamiya Epicentre,Ion Torrent,KAPA Biosystems 仪器/耗材 Applied Biosystems,Agilent,BD,Beckman coulter,Bio-Rad,GE,Perkin Elmer,Millipore,Parafilm,Uniequip
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