- SpeciesReactivityHuman
- SpecificityDetectshumanSyndecan-2/CD362indirectELISAs.IndirectELISAs, nocross-reactivitywithrecombinantmouse Syndecan-1,recombinanthuman(rh) Syndecan-3,orrhSyndecan-4isobserved.
- SourceMonoclonalRatIgG2BClone#305515
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenMousemyelomacelllineNS0-derivedrecombinanthumanSyndecan-2/CD362
Glu19-Gly144
Accession#AAH49836.1 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- FlowCytometry0.25µg/106cellsSeebelow
- CyTOF-readyReadytobelabeledusingestablishedconjugationmethods.NoBSAorothercarrierproteinsthatcouldinterferewithconjugation.
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Tkachenko,E.etal.(2005)Circ.Res.96:488.
- Oh,E.-S,andJ.R.Couchman(2004)Mol.Cells17:181.
- Essner,J.J.etal.(2006)Int.J.Biochem.CellBiol.38:152.
- Marynen,P.etal.(1989)J.Biol.Chem.264:7017.
- Choi,S.etal.(2005)J.Biol.Chem.280:42573.
- Park,H.etal.(2002)J.Biol.Chem.277:29730.
- Clasper,S.etal.(1999)J.Biol.Chem.274:24113.
- Modrowski,D.etal.(2000)J.Biol.Chem.275:9178.
- Halden,Y.etal.(2004)Biochem.J.377:533.
- Chen,L.etal.(2004)J.Biol.Chem.279:15715.
- EntrezGeneIDs:6383(Human);15529(Mouse)
- AlternateNames:CD362antigen;CD362;Fibroglycan;heparansulfateproteoglycan1,cellsurface-associated;Heparansulfateproteoglycancoreprotein;HSPG;HSPG1;HSPG1syndecanproteoglycan2;SDC2;SYND2;SYND2cellsurface-associatedheparansulfateproteoglycan1;syndecan2;Syndecan2;Syndecan-2
Background:
Syndecan-2,previouslyknownasfibroglycanorheparansulfateproteoglycan,isamemberofthesyndecanfamilyofType1transmembraneproteinscapableofcarryingheparansulfate(HS)andchondroitinsulfateglycosaminoglycans.Thefourvertebratesyndecansshowconservedcytoplasmicdomainsanddivergentextracellularportions(exceptforGAGattachmentsites).AmongtheSyndecans,Syndecan-2ismostsimilartoSyndecan-4(1‑3).HumanSyndecan-2issynthesizedasa201aminoacid(aa)coreproteinwithan18aasignalsequence,a126aaextracellulardomain(ECD),a25aatransmembraneregionanda32aacytoplasmictail(4).ThehumanECDofSyndecan-2containsthreeclosely-spacedconsensusSer-GlysequencesfortheattachmentofHSsidechains.Itshares76%,73%,87%,78%and63%aaidentitywiththeECDofmouse,rat,bovine, canineandchickenSyndecan-2,respectively.Thecytoplasmictailhasbothserineandtyrosinephosphorylationsites.Additionof20‑80disaccharidespersidechainaddsconsiderablytothesizeofthe22kDacoreprotein.Non-covalenthomodimerizationofSyndecan-2isdependentonthetransmembranedomain(5).Syndecan-2isexpressedincellsofmesenchymalorigin,neuronalandepithelialcells,andisthepredominantsyndecanexpressedduringembryonicdevelopment.Expressionisupregulatedinseveralcancercelllines(6).Afterinductioninmacrophagesbyinflammatorymediators,Syndecan-2selectivelybindsFGFbasic,VEGFandEGF(7).Syndecan-2expressedonhumanprimaryosteoblastsbindsGM-CSFandmayfunctionasaco‑receptor(8).ActivatedendothelialcellSyndecan-2specificallybindsIL-8andmayparticipateinpromotingneutrophilextravasationbyformingachemotacticIL-8grADIent(9).Typically,cytokine,chemokineandextracellularmatrixproteinbindingoccursthroughinteractionwithHSsidechains,buttheSyndecan-2extracellulardomaincanbindTGF-betadirectlyviaprotein-proteininteraction(10).
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