- Species ReactivityHuman
- SpecificityDetects human IL‑1 RAPL2/IL‑1 R9 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse IL‑1 RAPL2/IL‑1 R9 is observed. In Western blots, approximately 5% cross‑reactivity with recombinant human (rh) IL‑1 R7 is observed and less than 1% cross-reactivity with rhIL-1 R2 and rhIL-1 R8 is observed.
- SourcePolyclonal Goat IgG
- PurificationAntigen Affinity-purified
- ImmunogenMouse myeloma cell line NS0-derived recombinant human IL‑1 RAPL2/IL‑1 R9
Thr17-Glu356
Accession # Q9NP60 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Western Blot0.1 µg/mLRecombinant Human IL‑1 RAPL2/IL‑1 R9 Fc Chimera (Catalog # 1007-MR)
- Flow Cytometry2.5 µg/106 cellsSee below
- Immunohistochemistry5-15 µg/mLImmersion fixed paraffin-embedded sections of human liver and skin
- CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Andre, R. et al. (2005) J. Neurochem. 95:324.
- Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
- Jin, H. et al. (2000) Eur. J. Hum. Genet. 8:87.
- Carrie, A. et al. (1999) Nat. Genet. 23:25.
- Long Name:Interleukin 1 Receptor Accessory Protein-like 1
- Entrez Gene IDs:26280 (Human); 60367 (Mouse)
- Alternate Names:IL-1 R9; IL-1 RAPL2; IL-1R-9; IL1R9IL-1 receptor accessory protein-like 2; IL-1R9IL-1RAPL-2; IL1RAPL2; IL-1RAPL2; IL-1-RAPL-2; IL1RAPL-2IL1RAPL-2-related protein; interleukin 1 receptor 9; interleukin 1 receptor accessory protein-like 2; Three immunoglobulin domain-containing IL-1 receptor-related 1; TIGIRR-1; TIGIRR-1Interleukin-1 receptor 9; X-linked interleukin-1 receptor accessory protein-like 2
Background:
The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL-1 R4), IL‑18 R alpha(IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL‑18 R beta(AcPL/IL-1 R7), IL-1 RAPL1 (TIGIRR-2/IL-1 R8), and IL-1 RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL‑18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL-1 RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Its sequence predicts an 686 amino acid (aa) residue type I transmembrane glycoprotein with a 17 aa signal peptide, a 339 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 312 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL-1 RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL-1 RAPL1 and SIGIRR but not other family members (3). Human and mouse IL‑1 RAPL2 share approximately 95% aa sequence identity. Human IL‑1 RAPL2 is most homologous (63%) to IL‑1 RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL-1 RAPL2 has not been identified (1).
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