
- Species ReactivityHuman
- SpecificityDetects human Fibroblast Activation Proteinalpha /FAP in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human DPPIV is observed.
- SourcePolyclonal Sheep IgG
- PurificationAntigen Affinity-purified
- ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
Leu26-Asp760
Accession # Q12884 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
- LabelBiotin
- Western Blot0.5 µg/mLSee below
- Human Fibroblast Activation Proteinalpha /FAP Sandwich ImmunoassayReagent
- ELISA Capture (Matched Antibody Pair)2-8 µg/mLHuman Fibroblast Activation Protein alpha /FAP Antibody (Catalog # MAB3715)
- ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mLHuman Fibroblast Activation Protein alpha /FAP Biotinylated Antibody (Catalog # BAF3715)
- ELISA StandardRecombinant Human FAP Protein, CF (Catalog # 3715-SE)
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
- Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
- Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
- Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
- Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
- Entrez Gene IDs:2191 (Human); 14089 (Mouse)
- Alternate Names:170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin
Background:
FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.
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