
- SpeciesReactivityMouse
- SpecificityDetectsmouseCD27indirectELISAsandWesternblots.InWesternblots;doesnotcross-reactwithrecombinanthuman(rh)CD27,recombinantmouse(rm)CD40,rmGITR,rhDR6,rhTRAILR4,rmHVEM,rmCD30,rmRANK,rmFas,rmTRAILR2,rhTRADD,rm4-1BB,rmLTbeta R,rmOPG,rmNGFR,rmDR3,rmBAFFR,rhTRAILR3,rmTAJ,rmEDAR,rmOX-40,rmTWEAKR,rmTNFR1,rhRELT, orrmTRAIL R1.
- SourceMonoclonalRatIgG2AClone#137915
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenMousemyelomacelllineNS0-derivedrecombinantmouseCD27/TNFRSF7
Thr21-Arg182
Accession#P41272 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- FlowCytometry2.5µg/106cellsSeebelow
- CyTOF-readyReadytobelabeledusingestablishedconjugationmethods.NoBSAorothercarrierproteinsthatcouldinterferewithconjugation.
- Immunocytochemistry8-25µg/mLSeebelow
- MouseCD27/TNFRSF7SandwichImmunoassayReagent
- ELISACapture(MatchedAntibodyPair)2-8µg/mLMouseCD27/TNFRSF7Antibody(Catalog#MAB5741)
- ELISADetection(MatchedAntibodyPair)0.5-2.0µg/mLMouseCD27/TNFRSF7BiotinylatedAntibody(Catalog#BAM5741)
- ELISAStandardRecombinantMouseCD27/TNFRSF7FcChimeraProtein,CF(Catalog#574-CD)
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Croft,M.(2003)NatureReviewsImmunol.3:609.
- Croft,M.(2003)CytokineandGrowthFactorReviews14:265.
- Gravestein,L.A.etal.(1993)Eur.J.Immunol.23:943.
- Lens,S.M.etal.(1998)Semin.Immunol.10:491.
- Raman,V.S.etal.(2003)J.Immunol.171:5876.
- EntrezGeneIDs:939(Human);21940(Mouse);500318(Rat);102139476(CynomolgusMonkey)
- AlternateNames:CD27antigen;CD27molecule;CD27;MGC20393;S152CD27Lreceptor;TcellactivationantigenCD27;TcellactivationantigenS152;T14;TNFRSF7;TNFRSF7T-cellactivationantigenCD27;Tp55;Tumornecrosisfactorreceptorsuperfamilymember7;tumornecrosisfactorreceptorsuperfamily,member7
Background:
CD27isalymphocyte-specificmemberofthetumornecrosisfactorreceptorsuperfamily(TNFRSF)andisdesignatedTNFRSF7(1,2).MouseCD27CDNAencodesa250aminoacid(aa)residuetypeItransmembraneproteinwitha20aaputativesignalpeptide,a162aaextracellularregioncontainingthreeTNFRcysteine-richrepeats,a21aatransmembranedomainanda47aacytoplasmicregion(3).MouseandhumanCD27shareapproximately65%aminoacididentity.CD27existsashomodimersonthecellsurfaceviaanextracellulardisulfidebondinthemembrane-proximalregion.AsolubleformofCD27isalsoproducedduringtheimmuneresponseandisfoundinvariousbodyfluids(4).CD27isexpressedonsubsetsofTandBcells.TheexpressionofCD27isupregulateduponTcellactivation.AlthoughCD27appearstobeaMarkerforhumanmemoryBcells,itisonlyexpressedinasmallpopulationofmouseBcellsingerminalcentersandatsitesofB cellstimulation,suggestingthatmouseCD27maybeamarkerforactivatedBcells(5).CD27interactswithCD27ligand(alsonamedCD70andTNFSF7),whichisamemberoftheTNFligandsuperfamily.LigationofCD27onTcellsprovidescostimulatorysignalsthatarerequiredforTcellproliferation,clonalexpansionandthepromotionofeffectorTcellformation(1,2).LigationofCD27onBcellshasbeenshowntoinhibitterminaldifferentiationofactivatedmouseBcellsintoplasmacellsandenhancescommitmenttomemoryBcellresponses(5).
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