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BioBasic/10-250kDa Wide Range Blue-Red Two Color Protein Ladder, Prestained, 100ul/100ul/BZ0010R
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BioBasic/10-250kDa Wide Range Blue-Red Two Color Protein Ladder, Prestained, 100ul/100ul/BZ0010R
品牌 / 
BioBasic
货号 / 
BZ0010R
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数    量:
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Product Description:Product Features - Prestained Protein LadderBroad range (10-250 kD)Dual-Color or Tri-ColorHigh size precision and lot consistencyClean and sharp bandsHigh membrane binding affinityHigh storage stabilityBio Basic prestained protein ladder introductionBio Basic prestained protein ladder is designed for estimating the approximate size of separated proteins on SDS-PAGE gel. The ladders are a mixture of recombinant proteins ranging from 10 kD to 250 kD. Red or green bands at 70 kD and 25 kD provide easy references for molecular weight identification. The molecular weights of the prestained ladders are confirmed using the Tris-Glycine SDS-PAGE system with an accuracy of >95% by using unstained protein ladders. The protein ladders are highly stable with minimal band broadening during storage. Products are conveniently packaged and ready to use, with no heating, diluting or additional reducing agent required.Bio Basic Prestained protein ladder has high size precisionTo perform molecular weight (MW) size precision evaluation, both unstained and prestained ladders were resolved on a 4-15% gradient SDS-PAGE Gel (BioRad #4561083) followed by Coomassie BB staining (left panel). MWs of all protein bands were indicated at their migration positions (right panel). As seen in lane 3, 4 and 5, some prestained protein bands show significant MWs inconsistencies between BioRad’s and Thermo’s products. Similar inconsistent bands are also observed between two ladders from the same manufacturer (Thermo #26616 and #26619). Generally, unstained protein ladders are considered to be more precise. Here, two unstained ladders (Thermo #26630 and NEB #7703, a third-party’s product) were included as standard references (lane 1&2). When compared with unstained ladders, there are obvious imprecise prestained bands in both popular brands’ products. Interestingly, the MWs of Thermo’s prestained ladders (#26616 and #26619) do not match their own unstained protein ladder (#26630). There are no significant imprecise bands in the Bio Basic ladder (lane 6). It should be noted that presteined ladders were originally designed for approximative MW reference and therefore, neither represent an absolute standard.*Normally, all prestained protein ladders have lot-to-lot variation. This evaluation only represented the tested lot and did not intend to conclude a judgement of all other lots of listed brands’ products.**The evaluation was performed on Tris-Glycine buffer system. The mobility of prestained proteins can vary in different SDS-PAGE buffer systems.
Protein
Bio Basic Prestained protein ladder has high stabilityWe fully understand that storage stability is very important for bulk purchasers. Bio Basic prestained protein ladders have excellent stability and can be stored at frozen for long term. For storage stability test, BZ0010R was stored at -20°C and 37°C, respectively, for one month and resolved on 12.5% Laemmli SDS-PAGE gel. Compared with storage in -20°C, BZ0010R only slightly faded (lane 1&2) and little protein degradation occurred (lane 3&4) after one-month storage at 37°C. This result clearly showed that Bio Basic prestained ladder is highly stable even in such harsh condition.
Shipment and storageProducts are shipped on ice pack and should be store frozen upon receipt. Occasional thawing partly during shipping will not affect the quality and performance because our protein ladders are substantial stable in ambient temperature. We recommend to store ladders at -80°C for long term storage. When you subpackage, product should be equilibrated at 4°C overnight and further let it stay at room temperature until completely thawed. Mix thoroughly to make sure no insoluble precipitate occurs. If necessary, warm it at 37°C to dissolve any precipitate (no heat!). Aliquoting recommended. Store all aliquots as above or at -20°C for one year.11 bands: 10, 15, 20, 25 (Red), 35, 40, 50, 70 (Red), 100, 150, 250 kDa
Total Product Size:100ul
Individual Container Size:100ul
Number of Containers:1
Refrigeration Requirements:Freezer
Shipping Conditions:ICE
UNSPSC Code:41105337
UNSPSC Category:Protein Molecular Weight Markers
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gleason分级_123
隐幽羽2018-02-04
如题,最好有详解
http://www.medicalnewstoday.com/medicalnews.php?newsid=58498

NaturalProteinStopsDeadlyHumanBrainCancerInMice


ScientistsfromJohnsHopkinsandfromtheUniversityofMilanhaveeffectivelyproventhattheycaninhibitlethalhumanbraincancersinmiceusingaproteinthatselectivelyinducespositivechangesintheactivityofcellsthatbehavelikecancerstemcells.ThereportispublishedinNature.

Themostcommontypeofbraincancer-glioblastoma-ismarkedbythepresenceofthesestem-cell-likebraincells,which,insteadoftriggeringthereplacementofdamagedcells,formcancertissue.Stemcells,unlikeallothercellsinthebody,arecapableofformingalmostanykindofcellwhentheright"signals"triggertheirdevelopment.

Fortheirtreatmentexperiment,theresearchersreliedonaclassofproteins,bonemorphogenicproteins,thatcauseneuralstem-cell-likeclusterstolosetheirstemcellproperties,whichinturnstopstheirABIlitytodivide.

Firsttheypretreatedhumanglioblastomacellswithbonemorphogenicprotein4(BMP4),theninjectedthesetreatedcellsintomousebrains.Inmiceinjectedwithcellsthatwerenotpretreated,large,invasivecancersgrew.InthemicewithBMP4-treatedcells,nocancersgrewatall.Threetofourmonthsafterinjection,allmicethatgotuntreatedcellsdied,andnearlyallmicewithBMP4-treatedcellswerealive.

Next,thescientistsdeliveredslow-releaseBMP4-containing"beads"directlyintomousebrainswithimplantedglioblastomacells.Micethatgotemptybeadsdevelopedlargemalignanttumorsanddied.MicewithBMP4beadssurvivedmuchlonger,and80percentsurvivedfourmonthsaftercancercellimplants.

"OurideaistotreatpatientswithBMP4orsomethinglikeitrightaftersurgerytoremoveglioblastomainhopesofpreventingtheregrowthofthecancerandimprovingsurvivaltime,"saysAlessandroOlivi,M.D.,directoroftheDivisionofNeurosurgicalOncologyatHopkinsandacontributortothestudy.

OlivisaysclinicalstudiesusingBMP4couldbeginwithinayearand,ifsuccessful,drugtherapiescouldbeavailabletothepublicwithinthreetofouryears.

"ThiswasproofoftheideathatBMPscouldstopglioblastomabydepletingthestem-cell-likepopulationthatfeedsit,"saysHenryBrem,M.D.,chairmanoftheDepartmentofNeurosurgeryatHopkinsandacollaboratorinthestudy."Thisopensexcitingdoorstofutureresearchintotreatmentsandtherapiesforsuchadevastatingdisease."
从组织中粗提蛋白时加尿素是为了增加蛋白的溶解,是吧?一般用多大浓度呢?

但是尿素是否会导致天然蛋白的失活呢?通过透析除去尿素后蛋白能“复活”吗?
我现在在纯化天然蛋白时第一步层析使用的是苯基的疏水层析柱,样品和缓冲液的硫酸铵浓度为0.5M,PH在5.6左右,发现蛋白能结合到柱子上,但是在洗脱时采用梯度洗脱洗不下来,最后用水洗也洗不下来,后来我用少量的0.15M的氢氧化钠洗出一高峰,请问,这是因为我的蛋白结合的太牢吗?该怎样解决呢?
再就是疏水层析和PH值有这么大的关系吗?
谢谢!
我想做个目的蛋白的单抗,通过基因克隆、表达、纯化来得到目的蛋白,但是因为用的是原核表达系统,缺少翻译后的正常修饰,所以肯定与天然蛋白有区别,这样的话,作出来的单抗就不一定能够识别组织中的天然蛋白了,有什么方法可以减少他们的差异?也就是尽量让单抗可以识别天然蛋白。谢谢!
小弟用E.coli表达的一蛋白(一天然毒素的N段)制备了11株单抗,但没有一株能中和天然蛋白,这可急死我了.再拖下去就不能毕业了.导师天天催我建立诊断方法呢.大家给我出出主意!
如题,最近做天然蛋白WB,可是效果老不理想

所以请教一下高手
哪些食物中富含蛋白质 123
滋小味fA3D2018-02-05
见蛋白质的含量(每100克食物)如下:大米7克、面粉9克、黄豆36克、绿豆24克、豆腐7.4克、白菜2克、茄子2.3克、苹果0.4克、花生27克、猪肉9.5克、牛肉20克、人乳1.5克、牛乳3.3克、鸡蛋15克、鲤鱼17克、对虾21克。虽然人乳、牛乳、鸡蛋中的蛋白质含量较低,但它们所含的必需氨基酸量基本上与人体相符,所以营养价值较高,是膳食中最好的食品。
我是研一的新手,导师昨天说要分给我一个比较难的课题,蛋白质分离纯化。我们实验室主要是做微生物生物化学与分子生物学方面的,没有专门搞蛋白分离纯化的,应该是天然蛋白的分离纯化。天然蛋白质的分离纯化比较难,主要指什么方面比较难呢?仅仅蛋白质分离纯化是不是也不够作一篇硕士论文的工作量呢,是不是也应该有后续的工作或者前期的工作呢?蛋白质分离纯化方面的课题对未来就业有何帮助?谢谢大家!十分感激!
我将目的基因插入到pPIC9K中的EcoRI和NotI位点上,分泌表达得到的蛋白经信号肽切除后,在N端是不是还要比天然蛋白多8个氨基酸呀?请大家指点,谢谢。
有两题不解
天然蛋白质中不存在的氨基酸:同型半胱氨酸
在天然蛋白质的组成中,不含有的氨基酸是:瓜氨酸
我怎么觉得这两题一样呢