Contributor:SupryaJayadevDate:Mar.11,1991I.Lower,chloroformphase:1)Dryonrotovaporsystemwithhousevacuumlines.Itisnotnecessarytodrysamplecompletely,butsufficientlytoyieldafinalvolumeof~2mls.II.Upper,aqueousphase:1)Dryonrotovaporsystemwithvacuumpump.DryascompletelyaspossIBLe.a)Willbeleftwithwithanoilthatsmellsverybad(becauseoftheDMSO)2)ResUSPendin~2mlchloroform.III.Interphase:1)Dryasmuchaspossibleonrotovaporsystemwithhousevacuum.2)Resuspendemulsioninbenzeneanddrydownagainusingrotovaporandhousevacuum.3)Resuspendtheresultantwhitecrystallinelikepowderin~5mlsofchloroform.________________________________________________________________________1)RunallthreeoftheabovesamplesonaTLCwiththesolventsystem:chloroform-methanol-ammoniumhydroxide(60:35:8).Runsphingomyelinanddemethylatedsphingomyelinstandardsaswell.2)Stainwithiodineandpermanganate.-->Permanganateismoresensitivethaniodineinpickingupspots.-->DMSOwillbepredominantinboththeupperandlowerphases.3)ColumnpurifytheDMSM.