SECTIONS UnfixedcryostatorcoldFormolcalcium/gumsucrosefixedsections. SOLUTIONS a.Incubatingsolution. --acetylthiocholineiodide5mg --0.1Macetatebuffer(pH.6.0)6.5ml --0.1msodiumcitrate(2.94g/100ml)0.5ml --30mMcoppersulphate(0.58g/100ml)1.0ml --Distilledwater1.0ml --5mMpotassiumferricyanide(0.165g/100ml)1.0ml --Addinorder,mixingwellateachstage METHOD 1.Incubateinsolutiona.at37僀for15-120minutes. 2.Rinseindistilledwater. 3.CounterstaininHaematoxylin. 4.Dehydrate,clear.MountsectionsinDPX RESULTS CholinesteraseactivityRed/brown. NOTES i)Careshouldbetakenwhenusingcholinesteraseinhibitorsastheyareextremelytoxic. ii)Unfixedcryostatsectionsofrectalbiopsytakeonehour. iii)Unfixedcryostatsectionsofmusclebiopsytakeoneandhalftotwohours. NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.
