SUMMARY AND EXPLANATION
Prolactin (lactogenic hormone) is a single chain polypeptide hormone with a molecular weight of approximately 23,000 daltons. Prolactin is secreted from the anterior pituitary gland. Plasma and pituitary PRL levels are significantly greater in adult female than in male rats. This difference is thought to be brought about by ovarian steroids. A similar sex difference was found in pituitary PRL content in normal mice from 30 days of age. During and following pregnancy, prolactin, in association with other hormones, stimulates breast development and milk production. Hypersecretion of prolactin can be caused by pituitary tumors, hypothalamic diseases, hypothyroidm, renal failure, acute exercise and several medications.
| Catalog number | PR063F-100 |
| Product type | ELISA |
| Quantity | 96 Tests (12x8 breakable strip wells) |
| Standard range | 10-800 ng/ml |
| Analytical Sensitivity | 10 ng/ml |
| Sample volume | 10 µl/well |
| Species | Mouse or Rat |
| Application | The Calbiotech Mouse/Rat Prolactin ELISA kit is used for the quantitative measurement of prolactin in Mouse or Rat serum or plasma. |
| Principle | The Calbiotech Mouse/Rat Prolactin kit is based on a solid phase sandwich ELISA. The samples and diluted biotinylated anti-prolactin antibodies are added to the wells coated with polyclonal antibody to prolactin. Prolactin in samples binds to the anti-prolactin polyclonal antibody on the well and the biotinylated antiprolactin antibody binds to prolactin. Unbound protein and biotin conjugate are washed off by wash buffer. Diluted horseradish peroxidase conjugated streptavidin is added to each wells and the streptavidin is allowed to bind to the biotin in the complex. Unbound enzyme conjugate is washed off. Upon the addition of the substrate, the intensity of color is proportional to the concentration of prolactin in the samples. A standard curve is prepared relating color intensity to the concentration of the prolactin. |
| Storage and Stability | Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing. |
| Precautions | For research use only. Not for use in diagnostic procedures. |
| References | 1. Charlton HM, Speight A, Halpin DM, Bramwell A, Aheward WJ, Fink G. Prolactin measurements in normal and hypogonadal (hpg) mice: development and experimental studies. Endocrinology. 1983 Aug;113(2):548-8. 2. Duhau L, Grassi J, Grouselle D, Enjalbert A, Grognet JM. An enzyme immunoassay for rat prolactin: application to the determination of plasma levels.J Immunoassay. 1991; 12(2):233-50. 3. Gillet D, Ezan E, Ducancel F, Gaillard C, Ardouin T, Istin M, Menez A, Boulain JC, Grognet JM. Enzyme immunoassay using a rat prolactin-alkaline phosphatase recombinant tracer. Anal Chem. 1993 Jul 1; 65(13): 1779-84. 4. Leanos Miranda A, Quintal Alvarez MG, Cervera Castillo H, Blanco Favela F. Prolactin as an immunomodulator. Rev Alerg Mex. 1997 Sep-Oct; 44(5):116-23. 5. Ferrag F, Lebrun JJ, Touraine P, Nagano M, Dardenne M, Kelly PA. Prolactin and the immune system. Immunomethods. 1994 Aug; 5(1):21-30. |
PR063F-100-R3-RCType : PDF
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用双氧水去除过氧化物酶时,我是自己配的3%双氧水滴到片上而非将片浸入双氧水,这样可否?
用博士德的DAB染色,配制方法为:1mlH20中加入DAB、H2O2、TBS各一滴,我配制的时候发现将DAB加入H20时很容易沉淀,要震荡才能混匀,且将配好的DAB滴到玻片上时,DAB会结为很小块的微颗粒悬浮在液体中但并不染色,是否DAB有问题?
现在打算换中衫的试剂盒,代理商说中衫的试剂盒包括了二抗和DAB,想请教一下该试剂盒的具体内容有些什么?
万分感谢!
一、免疫组化试剂盒一般包括:
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别,所以购买时一定了解清楚:
1、一抗是做人的还是兔、鼠的组织;
2、检测系统是ABC/SP/非生物素/其他;
3、试剂盒的内容都有什么?有没有显色系统?
4、如果没有,自己根据前面的检测系统需配哪个显色系统。(其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配)
三、免疫组化抗体即指特异性一抗,是用来标记的指标,免疫组化过程的其他试剂一律另外配备。
另外:做实验时,如遇出结果困难,记得做阳性片对照,敢于怀疑检测系统或显色系统的问题。
上海好多的供应商的,具体还是看你选择了,其实试剂盒产品都差不多,什么远慕生物,古朵生物...好多的额

