| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
| Gene : | ACTB |
| Gene ID : | 60 |
| Uniprot ID : | P60709 |
| Alternative Name : | Actin, cytoplasmic 1, ACTB |
| Immunogen Information : | A partial length recombinant Beta actin protein (amino acids 1-200) was used as the immunogen for this antibody. |
B-actin (ACTB) has traditionally been regarded as an endogenous housekeeping gene and has been widely used as a reference gene/protein in quantifying expression levels in tumors. It supports fundamental cellular processes in healthy and diseased cells including cell adhesion, migration, cytokinesis and maintenance of cell polarity. However, B-actin is closely associated with a variety of cancers and accumulating evidence indicates that B-actin is de-regulated in liver, melanoma, renal, colorectal, gastric, pancreatic, esophageal, lung, breast, prostate, ovarian cancers, leukemia and lymphoma. This protein is generally found to be up-regulated in the majority of tumor cells and tissues.
Western blot analysis: 4-6 µg/ml:
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
1. Panda PK, Naik PP, Meher BR, Das DN, Mukhopadhyay S, Praharaj PP, Maiti TK,Bhutia SK. PUMA dependent mitophagy by Abrus agglutinin contributes to apoptosis through ceramide generation. Biochim Biophys Acta Mol Cell Res. 2018Mar;1865(3):480-495. doi: 10.1016/j.bbamcr.2017.12.002. Epub 2017 Dec 8. PubMedPMID: 2922947
2. Nayak TK, Mamidi P, Kumar A, Singh LP, Sahoo SS, Chattopadhyay S,Chattopadhyay S. Regulation of Viral Replication, Apoptosis and Pro-Inflammatory Responses by 17-AAG during Chikungunya Virus Infection in Macrophages. Viruses.2017 Jan 6;9(1). pii: E3. doi: 10.3390/v9010003. PubMed PMID: 28067803; PubMedCentral PMCID: PMC5294972.
3. Panigrahi B, Singh RK, Mishra S, Mandal D. Cyclic peptide-based nanostructuresas efficient siRNA carriers. Artif Cells Nanomed Biotechnol. 2018 Oct 12:1-11.doi: 10.1080/21691401.2018.1511574. [Epub ahead of print] PubMed PMID: 30311806.
4. Panigrahi B, Mishra S, Singh RK, Siddiqui N, Bal R, Mandal D. Peptidegenerated anisotropic gold nanoparticles as efficient siRNA vectors. Int J Pharm.2019 May 30;563:198-207. doi: 10.1016/j.ijpharm.2019.04.007. Epub 2019 Apr 3.PubMed PMID: 30953762.
5. Yavvari PS, Verma P, Mustfa SA, Pal S, Kumar S, Awasthi AK, Ahuja V, Srikanth CV, Srivastava A, Bajaj A. A nanogel based oral gene delivery system targetingSUMOylation machinery to combat gut inflammation. Nanoscale. 2019 Mar14;11(11):4970-4986. doi: 10.1039/c8nr09599j. PubMed PMID: 30839018.
6. Shahror RA, Linares G, Wang Y, Hsueh SC, Wu CC, Chuang DM, Chiang YH, Chen KY.Transplantation of Mesenchymal Stem Cells Overexpressing FGF21 FacilitatesCognitive Recovery and Enhances Neurogenesis in a Mouse Model of Traumatic Brain Injury. J Neurotrauma. 2019 Jul 12. doi: 10.1089/neu.2019.6422. [Epub ahead ofprint] PubMed PMID: 31298621.
| Subcellular location: | Cytoplasm, Nucleus |
| Post transnational modification: | (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
| BioGrid: | 106575.336 interactions. |
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用双氧水去除过氧化物酶时，我是自己配的3%双氧水滴到片上而非将片浸入双氧水，这样可否？
用博士德的DAB染色，配制方法为：1mlH20中加入DAB、H2O2、TBS各一滴，我配制的时候发现将DAB加入H20时很容易沉淀，要震荡才能混匀，且将配好的DAB滴到玻片上时，DAB会结为很小块的微颗粒悬浮在液体中但并不染色，是否DAB有问题？
现在打算换中衫的试剂盒，代理商说中衫的试剂盒包括了二抗和DAB，想请教一下该试剂盒的具体内容有些什么？
万分感谢！
一、免疫组化试剂盒一般包括：
1、特异性的一抗
2、免疫组化检测系统。
3、有的同时还具备显色系统。
二、不同的公司内容有差别，所以购买时一定了解清楚：
1、一抗是做人的还是兔、鼠的组织；
2、检测系统是ABC/SP/非生物素/其他；
3、试剂盒的内容都有什么？有没有显色系统？
4、如果没有，自己根据前面的检测系统需配哪个显色系统。（其他的辅助试剂如PBS缓冲液、抗原修复液等一般另卖或自配）
三、免疫组化抗体即指特异性一抗，是用来标记的指标，免疫组化过程的其他试剂一律另外配备。
另外：做实验时，如遇出结果困难，记得做阳性片对照，敢于怀疑检测系统或显色系统的问题。
上海好多的供应商的，具体还是看你选择了，其实试剂盒产品都差不多，什么远慕生物，古朵生物...好多的额
