- Oseltamivir acid
- Oseltamivir phosphate
- Peramivir
- Peramivir Trihydrate
- Nucleozin
- Oseltamivir
ZanamivirInfluenza A/B virus neuraminidases inhibitor |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
- View current batch:
- Purity = 98.00%
- COA (Certificate Of Analysis)
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
Cell experiment [1,2]: | |
Cell lines | HeLa-CD4-LTR-βgal cells and HeLa-tat cell, CV-1 cell |
Preparation method | The solubility of this compound in DMSO is >16.6mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reacting condition | 5 nM-10 mM |
Applications | In HeLa-CD4-LTR-βgal cells, zananivir interfered with cell-cell fusion with the IC50 of 0.19 mM. In CV-1 cell monolayers, zananivir (0.5 mM) reduced plaque area by 97%. Zananivir caused a concentration-dependent inhibition of hemadsorption. Zananivir (5 mM) strikingly reduced lipid mixing. zanamivir suppressed the growth of influenza A and B viruses with IC50 values of 5 nM-14 nM for laboratory-passaged strains and from 20 nM-16 μM for clinical isolates. |
Animal experiment [2]: | |
Animal models | Mice infected with influenza A |
Dosage form | Intranasal, 0.01-4 mg/kg |
Application | Intranasal zananivir treatment given prophylactically plus twice daily over days 0 to 3 in mice infected with influenza A reduced mortality and viral titres in lung homogenated and improved lung consolidation scores over 10 days. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Greengard O, Poltoratskaia N, Leikina E, et al. The anti-influenza virus agent 4-GU-DANA (zanamivir) inhibits cell fusion mediated by human parainfluenza virus and influenza virus HA[J]. Journal of virology, 2000, 74(23): 11108-11114. [2]. Elliott M. Zanamivir: from drug design to the clinic[J]. Philosophical Transactions of the Royal Society of London. Series B, 2001, 356(1416): 1885. |
Zanamivir Dilution Calculator
calculate
Zanamivir Molarity Calculator
calculate
Cas No. | 139110-80-8 | SDF | Download SDF |
Synonyms | N/A | ||
Chemical Name | (2R,3R,4S)-3-acetamido-4-(diaminomethylideneamino)-2-[(1R,2R)-1,2,3-trihydroxypropyl]-3,4-dihydro-2H-pyran-6-carboxylic acid | ||
Canonical SMILES | CC(=O)NC1C(C=C(OC1C(C(CO)O)O)C(=O)O)N=C(N)N | ||
Formula | C12H20N4O7 | M.Wt | 332.31 |
Solubility | ≥16.6mg/mL in DMSO | Storage | Store at -20°C |
Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. |
Zanamivir is a selective inhibitor of influenza A and B virus neuraminidases with IC50 values of 0.64-7.9nM [1].
Influenza A and B viruses are negative-strand RNA viruses. Neuraminidase is one of the two glycoproteins present on the surface of the virus. It is important to the pathogenicity and infectivity of the virus. The neuraminidases inhibitor, zanamivir, is a sialic acid analogue. It inhibits neuraminidases to cleave sialic acid on the surface of host cells and influenza viral envelope. In the in vitro assay, zanamivir suppresses the growth of influenza A and B viruses with IC50 values ranging from 5nM to 14nM for laboratory-passaged strains and from 20nM to 16μM for clinical isolates. In animal models infected with influenza A and B, treatment of zanamivir reduces the mortality and viral titres in lung homogenates and improves lung consolidation scores [1].
References:[1] Elliott M. Zanamivir: from drug design to the clinic. Philos Trans R Soc Lond B Biol Sci, 2001, 356(1416): 1885-93.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
这些染料的结构式基本是保密的。有些地方能查到,象维基上就有SYBR Green的结果式,但谁也不能肯定是否是正确的。
你的荧光染料具体是哪一种?你可以通过染色试验来决定是否有效啊?一般荧光染料染色后,都会呈现明亮的颜色,比一般同样颜色要亮的多。只要能染上、只要有亮度,就是没失效。
1.熔融温度为75,74,73度的样品是否是同一个产物?熔融温度为56度的是不是没有扩增产物?
2.为什么相同的标准品不同反应CT值差别这么大?是不是荧光染料降解?我用的是试剂盒中的扩增酶,还没用几次。
3.扩增曲线的形态是否说明标准品已经有降解?
4.另外,我原来做CDNA梯度稀释做的挺好,可是这个直接是RNA为标准品,我用一步法进行QRT-PCR。标准品梯度稀释线性效果不稳定,有的时候好,有的时候不好。请假大家有没有好的建议?
附件中有扩增曲线和熔融曲线,请大家帮忙分析,谢过了!
新建MicrosoftOfficeWord文档.docx(46.18k)
所以想请大家给推荐一个效果好的最好是国产的质粒提取试剂盒。
多谢!