![NewEast/Rab5 Activation Assay Kit [83701][20 assays]/83701](images/201910/1571694033440777269.jpg)
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ProductDescriptionSmallGTPasesareasuper-familyofcellularsignalingregulators.Rab5isamemberoftheRab-familyGTPases.Rab5isarate-limitingcomponentofthemachineryregulatingthekineticsofmembranetrafficintheearlyendocyticpathway. CurrentlythereisnodirectassaytomeasuretheactivationofRabGTPases. NewEastBiosciencesRab5ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesRab5-GTP,butnotRab5-GDP,andaRab5specificrabbitpolyclonalantibody.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility. Thisanti-Rab5-GTPmonoclonalantibodycanalsobeusedtomonitortheactivationofRab5incellsandintissuesbyimmunohistochemistry. NewEastBiosciencesRab5ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofRab5.Eachkitprovidessufficientquantitiestoperform20assays. AssayPrincipleNewEastBiosciencesRab5ActivationAssayKitbasesontheconfiguration-specificanti-Rab5-GTPmonoclonalantibodytomeasuretheactiveRab5-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngRab5activationassays.Briefly,anti-activeRab5mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingRab5-GTP.TheboundactiveRab5willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveRab5willbedetectedbyimmunoblotanalysisusinganti-Rab5specificrabbitpolyclonalantibody. ![]()
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