![NewEast/RheB Activation Assay Kit [81201][20 Assays]/81201](images/201910/1571693986592274876.jpg)
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ProductDescriptionSmallGTPasesareasuper-familyofcellularsignalingregulators.RheBisamemberoftheRas-superfamilyGTPases.RheBhasbeenshowntointeractwithC-Raf,Mammaliantargetofrapamycin(mTOR),TSC2,Ataxiatelangiectasiamutated(ATM),KIAA1303andAtaxiatelangiectasiaandRad3related. CurrentlythereisnodirectassaytomeasuretheactivationofRheBGTPases. NewEastBiosciencesRheBActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesRheB-GTP,butnotRheB-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility. Theseanti-RheB-GTPmonoclonalantibodycanalsobeusedtomonitortheactivationofRheBincellsandintissuesbyimmunohistochemistry. NewEastBiosciencesRheBActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofRheB.Eachkitprovidessufficientquantitiestoperform20assays. AssayPrincipleNewEastBiosciencesRheBActivationAssayKitbasesontheconfiguration-specificanti-RheB-GTPmonoclonalantibodytomeasuretheactiveRheB-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngRheBactivationassays.Briefly,anti-activeRheBmousemonoclonalantibodywillbeincubatedwithcelllysatescontainingRheB-GTP.TheboundactiveRheBwillthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveRheBwillbedetectedbyimmunoblotanalysisusinganti-RheBrabbitpolyclonalantibody. ![]() ![]()
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