PepMix™HSV2(R1)
ProductCode:PM-HSV2-R1
Poolof283peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughRibonucleoside-diphosphatereductaselargesubunit(Swiss-ProtID:P89462)ofHumanherpesvirus2forTcellassays(e.g.ELISPOT).
Amount:1vialcontaining15nmol(appr.25µg)perpeptide
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):Genitalherpes,Encephalitis,Cancer
DeliveryTime:2-5days
PepMix™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.
BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried
PepMix™HSV2(R1)
SelectedReferences
"InVitroStudiesoftheImpactofMaribavironCMV-SpecificCellularImmuneResponses"
Stacheletal.,JClinVirol. (2015)-PMID:26780109
"InductionOfBroad-basedImmunityandProtectiveEfficacybySelf-amplifyingmRNAVaccinesEncodingInfluenzaVirusHemagglutinin"
Brazzolietal.,JournalofVirology (2015)-PMID:26468547
"TargetingofNucleoproteintoChemokineReceptorsbyDNAVaccinationResultsinIncreasedCD8+-mediatedCrossProtectionAgainstInfluenza"
Baranowskaetal.,Vaccine(2015)-PMID:26387432
"CharacteristicsofImmuneMemory10-15yearsAfterPrimaryHepatitisBVaccination"
Hummeletal.,Vaccine(2015)-PMID:n.a.
"TCellsSpecificforDifferentLatentandLyticViralProteinsEfficientlyControlEpstein-BarrVirusTransformedBCells"
Nowakowskaetal.,Cytotherapy(2015)-PMID:26276009
"High-densityPrecultureofPBMCsRestoresDefectiveSensitivityofCirculatingCD8TCellstoVirus-andTumor-derivedAntigens"
Wegneretal.,Blood(2015)-PMID:26024876
"MetabolicRegulationofHepatitisBImmunopathologybyMyeloid-derivedSuppressorCells"
Pallettetal.,NatureMedicine(2015)-PMID:25962123
ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext
Testimonials
"Toestablishanovelroleformyeloidderivedsuppressorcells(Pallettetal,Nat.Med.2015)inchronicviralinfection,weutilisedthePepMixCEFPool(extended)aswellasacustomsynthesizedPepMixspanningthecoreregionofHBVgenotypeD.Whereas,theCEFpeptidepoolconsistsof32peptides,eachcorrespondingtoadefinedHLAclassI-restrictedT-cellepitopefromCytomegalo,Epstein-Barr,andInfluenzavirus,thelattercustomPepMixincluded15mericpeptidesoverlappingby10aminoacids.SpecificallythiscompositionenabledustomonitorboththeantiviralCD8+andCD4+TcellresponsesinchronicHBVinfectionandthenon-antigenspecificTcellsthatareknowntomediateimmunopathologyintheliver.OurentireexperiencewithJPT,fromordering/deliverytouseinthelabwasexcellent.Notonlywerethereagentsabletoperformreliablyandconsistentlyinvitrofrombatchtobatch,thecustomerandtechnicalsupportprovidedwascontinuallyavailableandefficientwhenneeded.JPTwillremainour"go-to"companyforpurchasingpeptides."
Dr.LauraJPallett,InfectionandImmunity,UniversityCollegeLondon,UK
PepMix™HSV2(R1)
| Application | AntigenspecificT-cellstimulation Immunemonitoring T-cellassays T-cellexpansion |
| ProteinName | Ribonucleoside-diphosphatereductase |
| Organism | Humanherpesvirus2(HHV2) |
| Indication/Topic | Cancer Encephalitis Genitalherpes Encephalitis |
| Numberofpeptides | 283 |
FurtherInformation:
| Amount | 1vialcontaining15nmol(appr.25µg)perpeptide |
| Specifications | Peptidescan(15merswith11aaoverlap) |
| Documentation | ProtocolandDatasheet |
| Swiss-ProtID | P89462 |
| Sequence | MANRPAASALAGARSPSERQEPREPEVA PPGGDHVFCRKVSGVMVLSSDPPGPAAY RISDSSFVQCGSNCSMIIDGDVARGHLR DLEGATSTGAFVAISNVAAGGDGRTAVV ALGGTSGPSATTSVGTQTSGEFLHGNPR TPEPQGPQAVPPPPPPPFPWGHECCARR DARGGAEKDVGAAESWSDGPSSDSETED SDSSDEDTGSETLSRSSSIWAAGATDDD DSDSDSRSDDSVQPDVVVRRRWSDGPAP VAFPKPRRPGDSPGNPGLGAGTGPGSAT DPRASADSDSAAHAAAPQADVAPVLDSQ PTVGTDPGYPVPLELTPENAEAVARFLG DAVDREPALMLEYFCRCAREESKRVPPR TFGSAPRLTEDDFGLLNYALAEMRRLCL DLPPVPPNAYTPYHLREYATRLVNGFKP LVRRSARLYRILGVLVHLRIRTREASFE EWMRSKEVDLDFGLTERLREHEAQLMIL AQALNPYDCLIHSTPNTLVERGLQSALK YEEFYLKRFGGHYMESVFQMYTRIAGFL ACRATRGMRHIALGRQGSWWEMFKFFFH RLYDHQIVPSTPAMLNLGTRNYYTSSCY LVNPQATTNQATLRAITGNVSAILARNG GIGLCMQAFNDASPGTASIMPALKVLDS LVAAHNKQSTRPTGACVYLEPWHSDVRA VLRMKGVLAGEEAQRCDNIFSALWMPDL FFKRLIRHLDGEKNVTWSLFDRDTSMSL ADFHGEEFEKLYEHLEAMGFGETIPIQD LAYAIVRSAATTGSPFIMFKDAVNRHYI YDTQGAAIAGSNLCTEIVHPASKRSSGV CNLGSVNLARCVSRQTFDFGRLRDAVQA CVLMVNIMIDSTLQPTPQCTRGNDNLRS MGIGMQGLHTACLKMGLDLESAEFRDLN THIAEVMLLAAMKTSNALCVRGARPFSH FKRSMYRAGRFHWERFSNASPRYEGEWE MLRQSMMKHGLRNSQFIALMPTAASAQI SDVSEGFAPLFTNLFSKVTRDGETLRPN TLLLKELERTFGGKRLLDAMDGLEAKQW SVAQALPCLDPAHPLRRFKTAFDYDQEL LIDLCADRAPYVDHSQSMTLYVTEKADG TLPASTLVRLLVHAYKRGLKTGMYYCKV RKATNSGVFAGDDNIVCTSCAL |
| ProteinLength(aa) | 1142 |
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
刚入这行，谢谢大家
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
