PvuII has a High Fidelity version PvuII-HF® (NEB #R3151).
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saverqualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
PvuII crystals (Ira Schildkraut and Joseph Bonventre, New England Biolabs)
Product Source
An E.coli strain that carries the PvuII gene from Proteus vulgaris (ATCC 13315).
This product is related to the following categories:
Restriction Endonucleases P R,
Time-Saver Qualified Restriction Enzymes Products
This product can be used in the following applications:
Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
核糖核酸酶A是内切核糖核酸酶,可特异地攻击RNA上嘧啶残基的3'端,切割与相邻核苷酸形成的磷酸二酯键。反应终产物是嘧啶3'磷酸及末端带嘧啶3'磷酸的寡核苷酸。无辅因子及二价阳离子存在时,核糖核酸酶A的作用可被胎盘RNA酶抑制剂(B1ackburn et al.1977)或氧钒—核糖核苷复合物(Puskas et al.1982)所抑制。核糖核酸酶A用途生化研究,测定核酸的结构RNase 保护检测去除非... 查看更多>