请使用支持JavaScript的浏览器! +,MultiplexAdaptor,UniqueDualIndexAdaptor,UMIAdaptor,AdaptorforMultiplexing,UniqueMolecularIdentifier,RNA-seqUMI,UMIsforRNA-seq,LibraryPrepUMIs,StubbyAdapter,xGenDualIndexUMIAdapters,Accel-NGSMIDs蚂蚁淘商城
商品信息
联系客服
NEB/NEBNext® Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1)/96 reactions/E7416S
郑重提醒:
无质量问题不接受退换货,下单前请仔细核对信息。
下单后请及时联系客服核对商品价格,订单生效后再付款。
NEB/NEBNext® Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1)/96 reactions/E7416S
品牌 / 
纽英伦
货号 / 
E7416S
美元价:
(友情提示:该价格仅为参考,欢迎联系客服询价!)
数    量:
免费咨询热线
4000-520-616

NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1) are built for more streamlined and informative NGS multiplexing, more accurate determination of transcript abundance, and removal of PCR duplicates. These index adaptors contain a pair of unique dual indices (i5 and i7) and one 11 nt UMI sequence. This set includes 96 unique dual index UMI adaptors, packaged in a single-use 96-well plate with a pierceable foil seal. The universal primer mixis supplied in a separate vial.

Combining UMI Adaptors with one of the NEBNext RNA library prep kits is simple and straightforward if you follow the protocols found in the UMI Adaptor-specific library prep kit manuals (listed below).

  • NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 or NEB #E7765)
  • NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770 or NEB #E7775)
  • NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400 or NEB #E7405)
  • NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850 or NEB #E7860)
  • NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E7750 or NEB #E7755)

The NEBNext line of Multiplex Oligos can be used with NEBNext sample prep products or other standard Illumina-compatible library preparation protocols. In addition to this set, index adaptors with UMIs are available for PCR-free and standard DNA-seq (NEB #E7395). For multiplexing without UMIs, NEBNext offers several options for added flexibility, including unique dual index primers (NEB #6440,NEB #6442, NEB #E6444, NEB #E6446), dual index primers (NEB #E7600andNEB #E7780), and single primer sets (in 12- and 96-index formats; NEB #E7335,NEB #E7500,NEB #E7710,NEB #E7730,NEB #E6609), as well as an option compatible with EM-seq™ and bisulfite sequencing (NEB #E7140).

The NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1) workflow shown below details the library barcoding steps of the library preparation process.

Figure 1: NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1) Workflow
Figure 2: NEBNext Unique Dual Index UMI Adaptors have equally efficient ligation and subsequent amplification
A. Universal Human Reference (UHR) RNA (Agilent® #740000) was used to prepare 96 libraries with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760) following NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) enrichment. Library yields were quantified (Agilent Tapestation 4200) and normalized by dividing the individual library yields by the average library yield across all 96 libraries. Adaptor ligation efficiency was robust with uniformity across all 96 Unique Dual Index UMI Adaptors. Each bar represents the average of at least 2 technical replicates.B. NEBNext Unique Dual Index UMI Adaptors have uniform clustering efficiency. 96 libraries were pooled and sequenced on the Illumina NextSeq® 500 (PE70). Each library is expected to contribute 1.04% of the total sequencing reads. Individual library yields were normalized by dividing the number of actual reads by the number of expected reads. No bias was observed across the 96 Unique Dual Index UMI Adaptor libraries.
Figure 3: NEBNext Unique Dual Index UMI Adaptors outperform competitor UMI adaptors
Libraries were produced using UHR RNA and the NEBNext Ultra II Directional RNA library prep kit (NEB #E7760) following NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) enrichment.A. The average library yield of triplicates is shown for three starting total RNA inputs: 10, 100, and 1,000 ng. During adaptor ligation either NEBNext Unique Dual Index UMI Adaptors or IDT xGen dual index adapters were used. Final library yields were quantified using the Agilent Tapestation 4200.B. Libraries were sequenced on the Illumina NextSeq 500 (PE70). Reads were downsampled to 5 million reads (seqtk) and aligned to the GRCh38 reference genome using HISAT 2.1. UMI information was added using fgbio (AnnotateBamWithUmis) and MarkDuplicates (Picard 2.18.2.1) was used to mark reads with UMI and determine duplication rate. NEBNext Unique Dual Index UMI Adaptor libraries produced libraries with a lower percentage of read duplicates.
Figure 4: Incorporating UMIs into analysis improves the accurate quantification of duplication rates
Triplicate libraries of three input amounts (10, 100, and 1000 ng) were produced using UHR RNA and the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760) following NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) enrichment and sequenced on the NextSeq 500. Reads were downsampled (seqtk) to 5 million reads and mapped to the GRCh38 reference genome (HISAT 2.1) and Fgbio (AnnotateBamWithUmis) was used to add the UMI information. Two approaches were used to calculate duplication: position and UMI information (green bars) or position alone (yellow bars) using MarkDuplicates (Picard 2.18.2.1).
Figure 5: Unique Dual Index UMI Adaptors allow for the correction of bias introduced with PCR amplification
Libraries were produced using UHR RNA and the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760) following NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) enrichment. Four technical replicate libraries were produced at three RNA inputs (10, 100 and 1,000 ng). Libraries were sequenced on the NextSeq 500 (PE70) and downsampled (seqtk)to 10 million reads for further analysis. To assess PCR bias, the same sequencing reads were compared using typical transcript abundance analysis (Salmon 0.9.1) against UMI deduplicated reads. UMI deduplication was performed by first mapping to the GRCh38 reference genome (HISAT 2.1) followed by Fgbio to add the UMI information. Non-duplicate reads were randomly ordered and converted to fastq before re-assessing transcript abundance using Salmon (0.9.1). The ratio of UMI-adjusted counts/raw read counts with no deduplication was calculated. Number of transcripts with at least 1 count in any library and with UMI/raw ratio ≥2 are plotted. In total, approximately 90,000 total transcripts were detected. An increased number of PCR cycles in library preparation correlates with the number of transcripts that shift upon UMI correction.
Figure 6: IGV visual representation of mapped reads adjusted utilizing UMIs in MDM4 (chr1:204,548.089-204,558,681)
Several isoforms of MDM4 locus showed >2X fold change in UMI/raw ratio in a 10 ng library sequenced on the NextSeq 500 (PE70). Reads that are considered to originate from unique molecules are shown in blue. Duplicate reads determined by UMI sequence and mapped read position (red bars) can be removed to improve accuracy of transcript abundance in downstream analysis.

This product is related to the following categories:
NEBNext® Multiplex Oligos (Adaptors & Primers),
Next Generation Sequencing Library Preparation
蚂蚁淘电商平台
ebiomall.com
公司介绍
公司简介
蚂蚁淘(www.ebiomall.cn)是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台, 该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁 淘搜索全球供应信息,找到合适的产品后在蚂蚁淘下单,然后蚂蚁淘的海外买手进行跨境采购、 运输到中国口岸,最后由蚂蚁淘国内团队报关运输给客户...
蚂蚁淘承诺
正品保证: 全球直采 在线追溯 蚂蚁淘所有产品都是自运营的,我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权; 同时客户可以通过订单详情查看到货物从厂方至客户的所有流程, 确保货物的来源; 正规报关,提供13%增值税发票。
及时交付: 限时必达 畅选无忧 蚂蚁淘的运营团队都是有着多年经验的成员,他们熟悉海外采购、仓储物流、报关等环节; 同时通过在线的流程监控,蚂蚁淘的进口速度比传统企业提高了50%以上, 部分产品甚至能做到7-10天到货,即蚂蚁淘的“时必达”服务。
轻松采购: 在线下单 简单省事 蚂蚁淘的价格是真实透明的,并且具有很大的价格优势,不需要繁杂的询价比价; 报价单与合同可以直接在线生成或打印;就像在京东购物一样, 您的鼠标点击几 次即完成在蚂蚁淘的采购,订单详情会告诉您所有进程。
售后申请: 耐心讲解 优质服务 蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时, 您可通过我的订单提交您的“申请售后”, 蚂蚁淘产品顾问会第一时间为您处理, 在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线:4000-520-616。
上海如吉生物科技发展有限公司在发布的核糖核酸酶A(牛胰)RNAase A供应信息,浏览与核糖核酸酶A(牛胰)RNAase A相关的产品或在搜索更多与核糖核酸酶A(牛胰)RNAase A相关的内容。 查看更多>
本页关键字:amresco去除核酸酶纸巾 amresco报价 amresco去除核酸酶纸巾,amresco报价。提供amresco去除核酸酶纸巾报价、咨询及技术服务,欢迎来电订购amresco去除核酸酶纸巾。中国试剂网经营实验试剂、实验耗材、实验仪器及实验技术服务。公司简介 Amresco 是全球著名的生化试剂生产商,其产品种类齐全,质量稳定,性价比好。深受广大用户的喜爱。Amresco公司来自美国,成立于 1976 年,为高质量生化试 查看更多>
上海研生实业有限公司所提供的去核酸酶试剂质量可靠、规格齐全,上海研生实业有限公司不仅具有精湛的技术水平,更有良好的售后服务和优质的解决方案,欢迎您来电咨询此产品具体参数及价格等详细信息! 查看更多>
核糖核酸酶A是内切核糖核酸酶,可特异地攻击RNA上嘧啶残基的3'端,切割与相邻核苷酸形成的磷酸二酯键。反应终产物是嘧啶3'磷酸及末端带嘧啶3'磷酸的寡核苷酸。无辅因子及二价阳离子存在时,核糖核酸酶A的作用可被胎盘RNA酶抑制剂(B1ackburn et al.1977)或氧钒—核糖核苷复合物(Puskas et al.1982)所抑制。核糖核酸酶A用途生化研究,测定核酸的结构RNase 保护检测去除非... 查看更多>
上海优予生物科技有限公司在发布的9001-99-4核糖核酸酶A(美仑);RNase A;核糖核酸酶I供应信息,浏览与9001-99-4核糖核酸酶A(美仑);RNase A;核糖核酸酶I相关的产品或在搜索更多与9001-99-4核糖核酸酶A(美仑);RNase A;核糖核酸酶I相关的内容。 查看更多>
2018-12-19
生工生物工程(上海)股份有限公司在发布的微球菌核酸酶供应信息,浏览与微球菌核酸酶相关的产品或在搜索更多与微球菌核酸酶相关的内容。 查看更多>
人靶向核糖核酸酶试剂盒,进口试剂盒http://www.aatbio.com.cn/人靶向核糖核酸酶试剂盒用于盛放检测化学成分、药物残留、病毒种类等化学试剂的盒子。一般医院、制药企业使用。核酸提取纯化类、蛋白检测类、RNA。abcamhttp://www.aatbio.com.cn/goodsid/fenleiyi/3200090_abcam/1.htmlabihttp://www.aatbio.com.cn/goodsid/fenle 查看更多>
人核糖核酸酶 ELISA人核糖核酸酶 ELISA属人ELISA试剂盒,是ELISA试剂盒中常用检测产品,的检测原理、产品报价及操作事项请咨询上海通善生物工程师,企业QQ 1465907913。属 人ELISA试剂盒规格 96T/48T包装 进口分装储存 2~8℃产品 美国供应商 上海通善生物特别说明 利用ELISA进行临床检验常见的样本一般包括血液(指血,静脉血),尿,粪便,脑脊液,胸腹水,前列腺液,精液,**分泌物等,这些样本收集的时 查看更多>
小鼠抗磷脂抗体(apa)elisa试剂盒使用注意事项:1.[jl40314] 小鼠抗磷脂抗体(apa)elisa试剂盒检测前,检查各种仪器,打开酶标仪,稳定20分钟左右。2.检测样本要澄清,不能含杂物,否则会直接影响结果。3.实验开始前要将试剂盒取出,室温放置30-40分钟,使各种试剂都恢复到室温,以使检测结果更为稳定。4.尽量做双标准实验,这样可以保证数据的准确性。5.底物具有毒性,终止液对皮肤有腐蚀性,要避免接触。6.[jl4031... 查看更多>
常见问题
蚂蚁淘所售产品均为正品吗?
蚂蚁淘的创始人兼CEO是钟定松先生,具有十年的从业经验,在业界享有良好的口碑; Ebiomall是跨境直采平台,我们直接从厂家采购,自己的团队负责国际物流和清关,中间没有第三方,蚂蚁淘承诺所售产品仅为正品,假一罚十。
下单后可以修改订单吗?
未确认状态的订单可以修改,打开“订单详情”页面,点击右上角的“修改订单”即可,若已审核确定,则订单无法修改。
商品几天可以发货?
现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。
订单如何取消?
如订单处于未确定状态,进入“我的订单"页面,找到要取消的订单,点击“取消订单”按钮。
可以开发票吗?
本网站所售商品都是正规清关,均开具13%正规发票,发票金额含配送费金额,另有说明的除外。
如何联系商家?
蚂蚁淘任何页面都有在线咨询功能,点击“联系客服”、“咨询”或“在线咨询”按钮,均可咨询蚂蚁淘在线客服人员, 或拨打4000-520-616,除此之外客户可在 联系我们页面找到更多的联系方式。
收到的商品少了/发错了怎么办?
同个订单购买多个商品可能会分为一个以上包裹发出,可能不会同时送达,建议查看订单详情是否是部分发货状态;如未收到,可联系在线客服或者致电4000-520-616。
退换货/维修需要多长时间?
一般情况下,退货处理周期为客户收到产品一个月内(以快递公司显示签收时间为准),包装规格、数量、品种不符,外观毁损、短缺或缺陷,请在收到货24小时内申请退换货;特殊商品以合同条款为准。
商品咨询
品牌分类
官网分类