Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Preservative:None. Product is not sterile, but may be sterile-filtered or a preservative may be added. Suggested Working Concentration or Dilution Range:1:100 - 1:400 for SARS-CoV-2 antibody ELISAs. Dilution should be optimized for assay of interest. Rehydration according to instruction yields 100% serum.Images & References
* Apparent antibody concentration quantified compared to a standard curve generated with SARS-CoV-2 Spike S1 Antibody (HC2001) – Genscript A02038-100 Figure legend: ELISA for SARS-CoV-2 antibodies from human serum was carried out as described in Amanat et al. (2020) with the following modifications:Coating/capture material: SARS-CoV-2 Spike Protein RBD (Genscript – Z03483-100) diluted to 2 µg/ml in PBS, and 50 µl (2 µg/well) was coated onto an ELISA plate overnight at 4˚C. Plates were blocked with 1% Bovine Serum Albumin (IgG-Free, Protease-Free) (001-000-162) in PBS + 0.05% Tween-20 (PBST). Samples: Human IgG sourced pre-August 2019 [ChromPure Human IgG, whole molecule (009-000-003)] was diluted to 250 µg/ml in 1% BSA/PBST, in triplicate, (100 µl/well). Human Serum sourced pre-August 2019 (009-000-243) was diluted 1:150 in 1% BSA/PBST in triplicate (100 µl/well). Human serum confirmed to be SARS-CoV-2 naive was drawn from seven volunteers. Human serum deemed to be SARS-CoV-2 antibody positive (~ 26-37 days after exposure to SARS-CoV-2, confirmed diagnosis) was drawn from four individuals. Volunteer-derived human serum samples were diluted 1:150 in 1% BSA/PBST (100 µl/well).Detection: HRP-Conjugated secondary antibody (109-035-064) was diluted in PBST at 1:20,000 and added to each well (100 µl/well). Plates were incubated with TMB (Moss TMBHK-1000) for 30 minutes at room temperature. TMB was detected at 620 nm using a plate reader.Standard/calibration curve:Standard curves were generated using Human IgG SARS-CoV-2 Spike S1 Antibody (HC2001) (Genscript A02038). The antibody was serially diluted (1:3) in 1% BSA/PBST, in triplicate, across the ELISA plate starting at 200 ng/ml (100 µl/well).Analysis: Graphpad Prism 8 was used to fit non-linear regression curves to a standard curve generated using the anti-SARS-CoV-2 antibody. Results and apparent antibody concentrations (µg/ml) in serum were interpolated from the curves generated.This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
Jackson总部位于美国宾夕法尼的兰卡斯特，距离费城大概有40英里。Jackson在1982年由科学家展开了包括在细胞生物学，蛋白质化学，微生物学，免疫学及产品开发实验研究。我们的目标是为世界各地的研究者能最大选择的提供最高质量的科学试剂以及最棒的技术支持和售后服务。蚂蚁淘向您提醒的是，Jackson产品仅用于研究，不用于诊断或治疗用途。作为美国著名的各种二抗生产公司之一，Jackson在全世界各国设有几十家代理。由于其产品效价高、种类全、价格低廉，因而成为全球各大药厂、试剂盒生产厂、大型实验室采购此类试剂的首选。其产品主要包括：各种纯化的二抗、标记的二抗（AMCA、Cy2、FITC、Cy3、TRITC、RRX、TR、Cy5、长臂生物素、辣根酶和碱磷酶标记）、金标试剂等。
Jackson二抗产品选购简介
为便于客户选购Jackson公司的各种二抗，特将选购时的注意事项摘译如下：
JacksonImmunoResearch的抗体产品线完全，有全球最大的二抗产品库，能提供多种应用类型和需要的二抗产品，其二抗产品本身也具有极高的产品质量和稳定性。以上述产品为例，其HRP酶标山羊抗小鼠二抗，WB实验的应用稀释比率为1:10000-1:200000，也就是说，购买的2ml二抗，实际应用时相当于20L-400L的最终使用液。另外，一次购买大包装，避免了不同批次之前的差异，降低了实验条件本身的误差。作为多家著名品牌的代理商，蚂蚁淘根据国内科研的现实需求，为客户选择最具性价比的二抗及血清产品免疫学解决方案。如果您对Jackson的产品感兴趣，请致电027-84454412到蚂蚁淘科技有限公司问询您所感兴趣的产品类型。
JacksonImmunoResearchLaboratories,INC.专业致力于亲和纯化的二抗和免疫球蛋白的生产及偶联。产品主要销往世界各地的大学和科研院所。该公司位于宾夕法尼亚州费城以西约40英里，始建于1982年，经过40年左右的发展，Jackson的二抗以种类丰富（物种齐全，标记多样，应用广泛）、性价比高、供货迅捷而受到更多科研学者和工业客户的青睐。
Jackson的产品主要有酶标二抗、荧光标记二抗、生物素标记二抗、胶体金标记二抗、封闭血清等等，其中JacksonHRP标记羊抗兔/羊抗鼠二抗，更是很多生产厂家的重要物料之一。苏州蚂蚁淘生物科技有限公司作为Jackson在中国的代理商，备有大量二抗现货，并能提供专业的售前咨询和完善的售后服务。
JacksonImmunoResearchLaboratories,INC.专业致力于亲和纯化的二抗和免疫球蛋白的生产及偶联。产品主要销往世界各地的大学和科研院所。该公司位于宾夕法尼亚州费城以西约40英里，始建于1982年，经过40年左右的发展，Jackson二抗以种类丰富（物种齐全，标记多样，应用广泛）、性价比高、供货迅捷而受到更多科研学者和工业客户的青睐。
Jackson的所有产品均具有极高的纯度和高超的品质，大部分二抗产品以冻干粉形式存在，易于保存和运输；作为二抗的实际生产商（非OEM商），产品的性价比也是非常高的，很多大厂家诸如T.F等也会从JacksonOEM产品。另外厂家完善的库存和供应体系以及高效稳定的纯化技术，保证了大部分二抗产品均能现货形式供应，不同批次的产品质量的稳定性也非常高。
Jackson的产品主要有酶标二抗、荧光标记二抗、生物素标记二抗、胶体金标记二抗、封闭血清等等，其中JacksonHRP标记羊抗兔/羊抗鼠二抗，更是很多生产厂家的重要物料之一。苏州蚂蚁淘生物科技有限公司作为Jackson在中国代理商，备有大量二抗现货，并能提供专业的售前咨询和完善的售后服务。
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