Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns. Tribioscience’s Corticosterone Competitive ELISA employs the competitive sandwich enzyme immunoassay technique to detect corticosterone concentrations in a variety of samples from plasmid, serum, and cell culture medium.
Kit Contents and Storage Conditions:
Part #
Catalog Number
TBS31002-01
TBS31002-05
Cortocosterone Microplate
TBS31002A
1x 96-well polystyrene microplate (12 strips of 8 wells) coated with polyclonal antibody
5x 96-well polystyrene microplate (12 strips of 8 wells) coated with polyclonal antibody
Corticosterone Standard
TBS31002B
1x 0.1mL, Corticosterone Standard (10 ng/mL)
5x 0.1mL, Corticosterone Standard (10 ng/mL)
Detecion A
TBS31002C
1x 3mL, a corticosterone-peroxidase conjugate in a special stabilizing solution
5x 3mL, A corticosterone-peroxidase conjugate in a special stabilizing solution
Detection B
TBS31002D
1x 3mL, a polyclonal antibody specific for corticosterone
5x 3mL, a polyclonal antibody sepcific for corticosterone
Assay Diluent
TBS31002E
1x 2mL, 5x concentrate Assay Diluent
1x 10mL, 5x concentrate Assay Diluent
10x Wash Buffer
TBS3000W
1x 10mL, 10x concentrated solution
1x 50mL, 10x concetrated solution
TMB Substrate
TBS3000T
1x 12mL, ultra-sensitive TMB substrate
5x 12mL, ultra-sensitive TMB substrate
Stop Solution
TBS3000S
1x 6mL, 2N sulfuric acid
1x 30mL, 2N sulfuric acid
Store the unopened kit between 2-8℃. Do not use kit past expiration date.
Example Standard Curve
Additional information
SIZE
5 x 96-well plate, 1 x 96-well plate
Specificity of the Assay and Recovery in Samples
Intra/Inter-Assay Precision
TBS31002_Corticosterone_ELISA_DataSheet
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