Bst 3.0 DNA Polymerase is anin silico designed homologue of Bacillusstearothermophilus DNA Polymerase I, LargeFragment engineered and fused to a novel nucleic acid binding domain for improved isothermalamplification performance and increased reversetranscription activity. Bst 3.0 DNA Polymerasecontains 5´→3´ DNA polymerase activity witheither DNA or RNA templates and strong stranddisplacement activity, but lacks 5´→3´ and 3´→5´exonuclease activity. Bst 3.0 DNA Polymerasedemonstrates robust performance even in highconcentrations of amplification inhibitors, including dUTP andfeatures significantly increased reverse transcriptaseactivity compared to Bst DNA Polymerase.
An example of the utility and speed offered by Bst 3.0 DNA Polymerase can be seen in this publication that explores the development of a novel digital LAMP assay: Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples.
Fast, single-enzyme RT-LAMP can be performed using Bst 3.0RT-LAMP was performed using indicated DNA polymerase and Jurkat total RNA and primers for two genes (ACTB, left; HMBS2, right). Fastest results were observed with a 2-enzyme system, Bst 2.0 and WarmStart RTx, but robust amplification was also observed using Bst 3.0 without additional RT. Bst LF, Bst 2.0 and competitor enzymes showed highly variable performance, with slow threshold times or reaction failure on one of the two targets.
Product Source
An E. coli strain that carries the engineered Bst 3.0 gene.
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