Based on the Vaccinia virus Capping Enzyme (VCE), the Vaccinia Capping System provides thenecessary components to add 7-methylguanylate cap structures (Cap-0) to the 5´end of RNA (1). In eukaryotes, these terminal cap structures are involved in stabilization (2), transport (3), and translation (4) of mRNAs. Enzymatic production of capped RNA is an easy way to improve the stability and translational competence of RNA used for invitro translation, transfection, and microinjection. Alternatively, use of labeled GTP in a reaction provides a convenient way to label any RNA containing a 5´ terminal triphosphate.
Vaccinia capping enzyme is composed of two subunits (D1 and D12). The D1 subunit carries three enzymatic activities (RNA triphosphatase, guanylyltransferase and guanine methyltransferase); all necessary for addition of a complete Cap-0structure, m7Gppp5"N to 5" triphosphate RNA (5,6). In vitro transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and the methyl donor, SAM. Capping is nearly 100% efficient and all capped structures are added in the proper orientation, unlike co-transcriptional addition of some cap analogs (7).
Figure 1. 5´ Cap StructureSchematic representation of mRNA 5´ cap structure indicating the 7-methylguanosine, shown in yellow, and the 5´ end of the mRNA, shown in blue. The 2´-O-methyl group present in Cap-1 and Cap-2 structures is shown in red.
Product Source
AnE. colistrain that carries an engineered His-tagged Vaccine capping gene.
This product is related to the following categories:
RNA Modification Products,
RNA Synthesis Products
This product can be used in the following applications:
DNase I 是一种核酸内切酶，降解双链或单链DNA，产生5-磷酸末端的单核苷酸及寡核苷酸，在Mg2+存在时，DNase I独立地作用每条DNA链，切割位点是随机分布，在Mn2+存在下,DNase I 作用于DNA双链的大致同一位置，产生钝末端或具1-2个核苷酸突起的DNA片段。来源：牛胰贮存条件：4℃应用：1、用切口平移法进行放射性标记时，可用DNase I在双链DNA上产生随机切口；2、在进行亚硫酸氢盐介导的诱变前，可用DNase... 查看更多>
核糖核酸酶A溶液产品名称：核糖核酸酶A溶液产品简介：上海通善生物是核糖核酸酶A溶液最权威的供应商，提供报价，咨询，技术服务，欢迎来电021-61806666咨询选购。产品库存：现货。产品价格：询价。供 应 商：通善生物。供应商地址：上海市闵行区金平路788弄166号。核糖核酸酶A溶液说明书：核糖核酸酶A溶液其它信息：货号产品名称级别K610-1L0.1X SSC BUFFER WITH 0.2% SDS超纯级K611-1L0.1X SS 查看更多>
