Trypsin-ultra™, Mass Spectrometry Grade is a serine endopeptidase. It selectivelycleaves peptide bonds C-terminal to lysineand arginine residues (1). Trypsin-ultra istreated with L-(tosylamido-2-phenyl) ethylchloromethyl ketone (TPCK) to inactivate anyremaining chymotryptic activity. It is modifiedby acetylation of the ε-amino groups of lysineresidues to prevent autolysis. Trypsin-ultra (TPCKtreated)cleaves at Lys-Pro and Arg-Pro bondsat a much slower rate than other amino acidresidues (2).
Digestion with Trypsin-ultra results in a high number of proteins identified, and sequence coverages as high as 90%
A 3-hour Trypsin-ultra digest of Pyrococcus furiosus using a 1:50 enzyme:substrate ratio, followed by ESI-MS, resulted in 418 proteins identified with individual protein sequence coverage as high as 90%.
Product Source
Isolated from bovine (Bos taurus) pancreas.
Reconstitution
Trypsin-ultra, Mass Spectrometry Grade should be reconstituted by the addition of 20–200 μl of high purity water. Rapid autolysis is a function of enzyme concentration.
This product is related to the following categories:
Proteases Products,
Proteome Analysis Products
This product can be used in the following applications:
核糖核酸酶A是内切核糖核酸酶,可特异地攻击RNA上嘧啶残基的3'端,切割与相邻核苷酸形成的磷酸二酯键。反应终产物是嘧啶3'磷酸及末端带嘧啶3'磷酸的寡核苷酸。无辅因子及二价阳离子存在时,核糖核酸酶A的作用可被胎盘RNA酶抑制剂(B1ackburn et al.1977)或氧钒—核糖核苷复合物(Puskas et al.1982)所抑制。核糖核酸酶A用途生化研究,测定核酸的结构RNase 保护检测去除非... 查看更多>