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GenWay/ACTH Ultra Sensitive lumELISA*/GWB-55CF27/ELISA_Kits
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4000-520-616
Description:
IntendedUse | TheGenWay,Inc.ACTHChemiluninescenceELISA(lumELISA)isanultrasensitivemethod(Lessthan1pg/mL)intendedforthequantitativedeterminationofACTH(AdrenocorticotropicHormone)inMouse/Ratplasma. |
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SummaryAndExplanation | AdrenocorticotropicHormone(ACTH)isa39-aminoacidpeptidehormone(MW=4500)secretedmainlybytheanteriorpituitarygland.Varioustypesofstressorpainperceivedinhigherlevelsofthebrainmodulatesecretionofthehypothalamicneurosecretoryhormone,corticotropinreleasinghormone(CRH).CRHstimulatespituitaryACTHsecretion.ThesecondpeptidethatmodulatesACTHsecretionisvasopressin(AVP).AVPsecretionisalsostimulatedbystressandactssynergisticallywithCRHtoincreaseACTHsecretioninthepituitaryportalcirculation. |
PrincipleOfTheTest | TheCBIACTHImmunoassayisatwo-sitelumELISA(ChemiluninescenceEnzyme-LinkedImmunoSorbentAssay)forthemeasurementoftheBIOLOGicallyactive39aminoacidchainofACTH.AgoatpolyclonalantibodytoACTH,purifiedbyaffinitychromatography,andamousemonoclonalantibodytoACTHarespecificforwell-definedregionsontheACTHmolecule.OneantibodyispreparedtobindonlytheCterminalACTH34-39andthisantibodyisbiotinylated.Theotherantibodyispreparedtobindonlythemid-regionandN-terminalACTH1-24andthisantibodyislabeledwithhorserADIshperoxidase[HRP]fordetection.Inthisassay,calibrators,controls,orsamplesaresimultaneouslyincubatedwiththeenzymelabeledantibodyandabiotincoupledantibodyinastreptavidin-coatedmicroplatewell.Attheendoftheassayincubation,themicrowelliswashedtoremoveunboundcomponents.Upontheadditionoftheluminolsubstrate,theenzymeactivityintheenzyme-boundfractionisdirectlyproportionaltotheconcentrationoftheACTHinthesample.Astandardcurveispreparedrelatinglightunit(RLU)totheconcentrationoftheACTH.ConcentrationsofACTHpresentinthecontrolsandsamplesaredetermineddirectlyfromthiscurve. |
Sensitivity | Thesensitivityofthisassayisdefinedasthesmallestsinglevalue,whichcanbedistinguishedfromzeroatthe95%confidencelimit.TheCBIACTHlumELISAhasacalculatedsensitivityofLessthan1pg/mL. |
AdditionalInformation:
Name | ACTHUltraSensitivelumELISA* |
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RelatedProductNames | ACTHUltraSensitivelumELISAELISAKit |
MolecularWeight | 0.5 |
IntendedUse | ResearchUseOnly |