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GenWay/Rabbit Transferrin ELISA Quantitation Kit/GWB-6E0BA8/96 well
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4000-520-616
Description:
ShortDescription:Transferrin(TX)isametal-combiningproteinthatreversIBLybindstoacid-solubleironinplasma.Itfunctionstotransportirontothebonemarrowandtotissuestorageorganssuchastheliver.Transferrinalsoparticipateintheregulationandcontrolofironabsorptionandprotectsagainstironintoxication.Likehaptoglobin,thecarrierofhemoglobin,transferrinissynthesizedintheliver,butunlikehaptoglobintransferrinisreturnedtothecirculationafterunloADIngitsironinthereticuloendothelialsystem.ThisELISAkitcanbeusedtomeasuretransferrininserum,tissueextractsandotherBIOLOGicalfluids.AdditionalInformation:
| Name | RabbitTransferrinELISAQuantitationKit | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RelatedProductNames | RabbitTransferrinELISAKit;Transferrin;Siderophilin;Beta-1-metal-bindingglobulinRabbitTransferrinTF | ||||||||||||||||
| Geneid | 100009267 | ||||||||||||||||
| AliasSymbols | SEROtransferrin,transferrin,Beta-1metal-bindingglobulin,TF,Siderophilin | ||||||||||||||||
| ProteinName | Serotransferrin | ||||||||||||||||
| DescriptionofTarget | TransferrinsareironbindingtransportproteinswhichcanbindtwoFe3+ionsinassociationwiththebindingofananion,usuallybicarbonate.Itisresponsibleforthetransportofironfromsitesofabsorptionandhemedegradationtothoseofstoragea | ||||||||||||||||
| SwissprotID | P19134 | ||||||||||||||||
| SpeciesReactivity | Rabbit | ||||||||||||||||
| AssayInfo | AssayMethodology:QuantitativeSandwichELISA | ||||||||||||||||
| Sensitivity | 0.892ng/ml | ||||||||||||||||
| KitRange | 3.125ng/ml-100ng/ml | ||||||||||||||||
| Homology | Rabbit | ||||||||||||||||
| NCBIAccNumber | NP_001054.1 | ||||||||||||||||
| StandardRange | ReferToManual | ||||||||||||||||
| SampleType | Serum,plasmaandotherbiologicalfluids | ||||||||||||||||
| ProductFormat | InSolution | ||||||||||||||||
| Specimen/Volumes | ReferToManual | ||||||||||||||||
| SwissProtNumber | P02787 | ||||||||||||||||
| Applications | ELISA | ||||||||||||||||
| ReconstitutionandStorage | 1.DILUENT The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C. 2.WASHSOLUTION The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C. 3.ENZYME-ANTIBODYCONJUGATE Undilutedhorseradishperoxidaseanti-TXconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours. 4.CHROMOGEN-SUBSTRATESOLUTION TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 5.STOPSOLUTION TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 6.ANTI-RABBITTRANSFERRINELISAMICROPLATE Anti-RabbitTXcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack. 7.RABBITTRANSFERRINCALIBRATOR ThelyophilizedRabbitTransferrincalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours. | ||||||||||||||||
| Reactivity | Rabbit | ||||||||||||||||
| KitSize | 96well | ||||||||||||||||
| KitPrinciple | GenWayBiotechTransferrinELISAKit(Rabbit)(GWB-6E0BA8)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforSerotransferrinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Serotransferrin-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleSerotransferrincapturedinthewell. | ||||||||||||||||
| KitComponent |
| ||||||||||||||||
| KitDuration | 80Minutes | ||||||||||||||||
| Storage | Refertokitinstructionsforcomponentstorage | ||||||||||||||||
| LeadTime | Domestic:within1weekdelivery International:1week | ||||||||||||||||
| IntendedUse | ResearchUseOnly | ||||||||||||||||
| AdditionalInformation | IntendedUse:TheTransferrintestkitisahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringTransferrininbiologicalsamplesofrabbit. | ||||||||||||||||
| :: | Reagents: 1.DILUENTCONCENTRATE(RunningBuffer) Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer. 2.WASHSOLUTIONCONCENTRATE Onebottlecontaining50mlofa20Xconcentratedwashsolution. 3.ENZYME-ANTIBODYCONJUGATE100X Onevialcontaining150ulofaffinitypurifiedanti-RabbitTransferrinantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer. 4.CHROMOGEN-SUBSTRATESOLUTION Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3. 5.STOPSOLUTION Onevialcontaining12ml0.3Msulfuricacid. WARNING:Avoidcontactwithskin. 6.ANTI-RABBITTRANSFERRINELISAMICROPLATE Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-RabbitTX. 7.RABBITTRANSFERRINCALIBRATOR OnevialcontainingalyophilizedRabbitTransferrincalibrator. |
