商品信息
联系客服
郑重提醒:
无质量问题不接受退换货,下单前请仔细核对信息。
下单后请及时联系客服核对商品价格,订单生效后再付款。
GenWay/Mumps IgG ELISA/GWB-6F0E1E/ELISA_Kits
免费咨询热线
4000-520-616
Description:
| IntendedUse | TheGenWayMumpsIgGAntibodyELISATestKithasbeendesignedforthethedetectionandthequantitativedeterminationofspecificIgGantibodiesagainstMumpsvirusinserumandplasma.FurtherapplicationsinotherbodyfluidsarepossIBLeandcanberequestedfromtheTechnicalServiceofGenWay.Thisassayisintendedforresearchuseonly. |
|---|---|
| GeneralInformation | Mumps(Parotitis)isacommoncontagiousdiseasewithrelativelymoderatesymptomsduringchildhood,butincreasingcomplications,whenadultsareinfected.ThecausativeagentofmumpsisavirusoftheParamyxoviridaefamily.Thevirusnormallyinfectschildrenattheageof4to10.Theinfectionismainlytransmittedbytheairborneroute,butisalsospreadbyvariousobjectscontaminatedbytheindividual’ssaliva.Thediseaseshowsaseasonalprevalencewiththegreatestincidenceinwinterandspring.Bothamumpsinfectionorvaccinationleadtoapersistentimmunity.Thetypicalsymptomassociatedwithamumpsinfectionisa&BDquo;parotitis“(swellingoftheparotidglands).AdditionallypathologicalinvolvementoftheCNSandvariousglandularorgans(pancreas,thymus,thyroids)isatypicalfeatureofmumps.Amumpsinducedmeningitisisoneofthemostfrequentmanifestationsofthediseasewhichcanalsoappearwithoutparotitis.Thepublisheddataaboutthepresenceofmeningitisvarybetween1.4%and66%oftheillindividuals.Inmostcasestherefollowsreconvalescencewithoutcomplications.TherecognitionofMumpsinthelaboratoryismostlydonebydetectionoftheinfectiousagentitselforbythedeterminationofvirus-specificantibodies.TheSEROdiagnosisplaysamajorpart.Besidestheclassicalmethodslikecomplementfixation,hemagglutinationandneutralisationtestshavebeenintroducedaseriesofmodernassayslikeimmunofluorescence,rADIoimmunoassayandenzymeimmunoassay.TheimmunityofanindividualismonitoredbyanIgGELISA.IncreasingIgGtitersarehelpfulinthedeterminationofthecausativeagentbecausecrossreactionswithParainfluenza-Virustype2mayleadtoawronginterpretationofthereports.Intheearlystageofthedisease,significantIgMtitersmaybemonitored. |
| PrincipleoftheTest | TheGenWayMumpsIgGantibodytestkitisbasedontheprincipleoftheenzymeimmunoassay(EIA).Mumpsantigenisboundonthesurfaceofthemicrotiterstrips.Dilutedsampleserumorready-to-usestandardsarePipettedintothewellsofthemicrotiterplate.AbindingbetweentheIgGantibodiesoftheserumandtheimmobilizedMumpsantigentakesplace.Afteraonehourincubationatroomtemperature,theplateisrinsedwithdilutedwashsolution,inordertoremoveunboundmaterial.Thenready-to-useanti-human-IgGperoxidaseconjugateisaddedandincubatedfor30minutes.Afterafurtherwashingstep,thesubstrate(TMB)solutionispipettedandincubatedfor20minutes,inducingthedevelopmentofabluedyeinthewells.Thecolordevelopmentisterminatedbytheadditionofastopsolution,whichchangesthecolorfrombluetoyellow.Theresultingdyeismeasuredspectrophotometricallyatthewavelengthof450nm.TheconcentrationoftheIgGantibodiesisdirectlyproportionaltotheintensityofthecolor. |
AdditionalInformation:
| Name | MumpsIgGELISA |
|---|---|
| RelatedProductNames | MumpsIgGELISA |
| IntendedUse | ResearchUseOnly |

