Jackson公司315-036-046/PeroxidaseAffiniPureF(ab')₂Frag现货价格低
0.5ml
Target: Mouse
Host: Rabbit
AntibodyFormat: F(ab')₂Fragment
Specificity: IgG,FcγFragmentSpecific
MinimalCrossReactivity: HumanSerumProteins
Conjugate: HorserADIshPeroxidaseProductCategory: F(ab')₂FragmentAffinity-PurifiedAntibodies
Clonality: Polyclonal
F(ab')2 fragmentantibodiesaregeneratedbypepsindigestionofwholeIgGantibodiestoremovemostoftheFcregionwhileleavingsomeofthehingeregion.F(ab')2 fragmentshavetwoantigen-bindingFabportionslinkedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisabout110kDa.Theyareusedforspecificapplications,suchastoavoidbindingofsecondaryantibodiestolivecellswithFcreceptorsortoProteinAorProteinG.
Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswiththeFcportionofmouseIgGheavychainbutnotwiththeFabportionofmouseimmunoglobulins.NoantibodywasdetectedagainstmouseIgMornon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithhumanserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.
PhysicalState: Freeze-driedsolid
Storage:
Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate: oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.
Purity: Theantibodywaspurifiedfromantiserabyacombinationofpepsindigestionandimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.FcfragmentsandwholeIgGmoleculeshavebeenremoved.
Buffer: 0.01MSodiumPhosphate,0.25MNaCl,pH7.6
StABIlizer: 15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative: None(Warning:Useofsodiumazideasapreservativewillsubstantiallyinhibittheenzymeactivityofhorseradishperoxidase.)
SuggestedWorkingConcentrationorDilutionRange:
1:500-1:5,000forimmunohisto/cytochemistry
1:5,000-1:100,000forELISAandWesternblottingwithchromogenicsubstrates
1:10,000-1:200,000forWesternblottingwithECLsubstrates
Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.
HorseradishPeroxidase
Horseradishperoxidase(HRP)conjugatesarepreparedbyamodifiedNakaneandKawaoiprocedure(J.Histochem.Cytochem.1974. 22,1084).Peroxidaseconjugatesarecommonlyusedforimmunohistochemistry,Westernblotting,andELISA.Affinity-purifiedanti-horseradishperoxidaseandconjugatesareavailablefordetectionofhorseradishperoxidaseantigenorforsignalamplificationofHRP-containingreagents.Forimmunostainingofmammaliancells,anadvantageofusinganti-horseradishperoxidaseisreducedbackground,sincetheantibodydoesnotrecognizetheendogenousperoxidase-likeenzymesfoundinthosecells.
美国JacksonImmunoResearchLaboratories,Inc.专业致力于亲合纯化的第二抗体和纯化的免疫球蛋白的生产和偶联。其产品深受广大研究人员的认可,销售范围遍布全球,领域包括植物,动物和生物医学研究。公司成立于1982年,公司创业者具有深厚的专业背景:细胞生物学,蛋白化学,微生物学,免疫学。公司自创立初,就将为其他研究人员提供最新最全的二抗作为公司的持续发展目标。