Jackson公司111-295-144/RhodamineRed-X-AffiniPureGoatAn现货价格低
1.5mg
Target: Rabbit
Host: Goat
AntibodyFormat: WholeIgG
Specificity: IgG(H+L)
MinimalCrossReactivity: Human,Mouse,RatSerumProteins
Conjugate: RhodamineRed™-X(RRX)ProductCategory: WholeIgGAffinity-PurifiedAntibodies
Clonality: Polyclonal
WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.
Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculerabbitIgG.Italsoreactswiththelightchainsofotherrabbitimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithhuman,mouseandratserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.
PhysicalState: Freeze-driedsolid
Storage:
Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate: oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.
Purity: Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer: 0.01MSodiumPhosphate,0.25MNaCl,pH7.6
StABIlizer: 15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative: 0.05%SodiumAzide
SuggestedWorkingConcentrationorDilutionRange:
1:50-1:200formostapplications
Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.
RhodamineRed™-X(RRX)
Amax: 570 Emax: 590nm
RRX(RhodamineRed-X)conjugateshaveapeakofexcitationat570nmandapeakofemissionat590nm.AlthoughTRITChasbeenusedtrADItionallywithFITCfordoublelabeling,bettercolorseparationisachievedbyusingRRXorAlexaFluor®594.RhodamineRed-Xisparticularlyusefulfor3-and4-colorlabelingwithDyLight405,AlexaFluor®488,andAlexaFluor®647byusingaconfocalmicroscopeequippedwitha405nmlaserandakrypton/argonlaser.FluorescencefromRRXliesaboutmidwaybetweenthatofAlexaFluor®488andAlexaFluor®647,anditshowslittleoverlapwitheitherdye.Thekrypton-argonlaseremitslinesat488nm,568nm,and647nm,whichareoptimalforexcitingAlexaFluor®488,RRX,andAlexaFluor®647,respectively.Byaddinga405nmlaseranda420nmemissionfilter,4-colorlabelingispossIBLeusingDyLight405-conjugatedsecondaryantibodiesfromJIR(Figure5).Theseparationbetweenallfourdyesisperfectfor4-colorlabeling,andallfourdyesareverybright.
美国JacksonImmunoResearchLaboratories,Inc.专业致力于亲合纯化的第二抗体和纯化的免疫球蛋白的生产和偶联。其产品深受广大研究人员的认可,销售范围遍布全球,领域包括植物,动物和生物医学研究。公司成立于1982年,公司创业者具有深厚的专业背景:细胞生物学,蛋白化学,微生物学,免疫学。公司自创立初,就将为其他研究人员提供最新最全的二抗作为公司的持续发展目标。