Jackson公司209-545-097/AlexaFluor®488AffiniPureMouse现货价格低

1.0mg

Target: Human
Host: Mouse
AntibodyFormat: WholeIgG
Specificity: IgG,F(ab')₂fragmentspecific

MinimalCrossReactivity: Bovine,Horse,MouseSerumProteins

Conjugate: AlexaFluor®488
ProductCategory: WholeIgGAffinity-PurifiedAntibodies
Clonality: Polyclonal

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswiththeF(ab')2/FabportionofhumanIgG.Italsoreactswiththelightchainsofotherhumanimmunoglobulins.NoantibodywasdetectedagainsttheFcportionofhumanIgGoragainstnon-immunoglobulinserumproteins.Theantibodyexhibitsinherentminimalcross-reactiontomouseserumproteinsandhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithbovine,horseandmouseserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies

PhysicalState: Freeze-driedsolid 
Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing. 
Expirationdate: oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity: Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads. 
Buffer: 0.01MSodiumPhosphate,0.25MNaCl,pH7.6 
StABIlizer: 15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free) 
Preservative: 0.05%SodiumAzide 

SuggestedWorkingConcentrationorDilutionRange:

1:100-1:800formostapplications

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempiricall

AlexaFluor®488

Amax: 493 Emax: 519nm 

AlexaFluor®488-conjugatedantibodiesabsorblightmaximallyat493nmandfluorescewithapeakaround519nm.InaqueousmountingmediatheyarebrighterthanFITC,Cy2,andDyLight488.AlexaFluor®488conjugatesarerecommendedformaximumsensitivityforallimmunofluorescenceproceduresrequiringagreen-fluorescingdye,exceptforprotocolsthatincludemountinginplasticmountingmedia.

美国JacksonImmunoResearchLaboratories,Inc.专业致力于亲合纯化的第二抗体和纯化的免疫球蛋白的生产和偶联。其产品深受广大研究人员的认可，销售范围遍布全球，领域包括植物，动物和生物医学研究。公司成立于1982年，公司创业者具有深厚的专业背景：细胞生物学，蛋白化学，微生物学，免疫学。公司自创立初，就将为其他研究人员提供最新最全的二抗作为公司的持续发展目标。