Invitrogen™D368/Di-4-ANEPPDHQ现货价格低
1mg
ANEPdyesaremoleculesthatfluoresceinresponsetoelectricalpotentialchangesintheirenvironment.Thesearefast-responseprobesthatoperatebymeansofachangeintheirelectronicstructure,andconsequentlytheirfluorescenceproperties,inresponsetoachangeinthesurroundingelectricfield.Theiropticalresponseissufficientlyfasttodetecttransient(millisecond)potentialchangesinexcitablecells,includingsingleneurons,cardiaccells,andintactbrains.However,themagnitudeoftheirpotential-dependentfluorescencechangeisoftensmall;fast-responseprobestypicallyshowa2-10%fluorescencechangeper100mV.FurThermore,thesedyesdisplayapotential-dependentshiftintheirexcitationspectra,thuspermittingthequantitationofmembranepotentialusingexcitationratiomeasurements.
Potential-SensitiveANEPDyeSpecifications:
•Cationicmolecule;exhibitsverylowinternalizationandgoodsignal:noiseratioandisusefulforvisualizingcholesterol-enrichedlipiddomainsinmodelmembranes
•Ex/Emmaximaboundtomodelphospholipidmembranesare~465/635nm(butspectralpropertiesarehighlydependentonenvironment)
•Nonfluorescentuntilboundtomembranes
•Solubleinethanol,DMSO,andDMF(di-2-ANEPEQisawater-solubleANEPdye)
•Dyeisintroducedintocellsbydirectadditionofstocksolutiontocellculturemedium,byusingPluronic®F-127,orbyretrogradelabeling
•Fast-responseprobe,suitablefordetectingsubmillisecondmembranepotentialchanges
ApplicationsforPotentiometricProbes
Theplasmamembraneofacelltypicallyhasatransmembranepotentialofapproximately-70mV(negativeinside)asaconsequenceofK+,Na+,andCl-concentrationgrADIentsthataremaintainedbyactivetransportprocesses.Potentiometricprobesofferanindirectmethodofdetectingthetranslocationoftheseions.
Increasesanddecreasesinmembranepotential-referredtoasmembranehyperpolarizationanddepolarization,respectively-playacentralroleinmanyphysiologicalprocesses,includingnerve-impulsepropagation,musclecontraction,cellsignalingandion-channelgating.Potentiometricprobesareimportanttoolsforstudyingtheseprocesses.
FindMoreANEPDyes
WeofferANEPdyesinvariousforms.ReviewFast-ResponseProbes-Section22.2intheMolecularprobes®Handbookformoreinformationontheseprobes.
ForResearchUseOnly.Notforhumanoranimaltherapeuticordiagnosticuse.
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