Invitrogen™A10196/QDOT625STREPTAVIDINCONJUGAT现货低价
200ml
Qdot®625StreptavidinConjugatecomprisesabiotin-bindingprotein(streptavidin)covalentlyattachedtoafluorescentlabel(Qdot®nanocrystal).Streptavidinhasaveryhighbindingaffinityforbiotin,andaconjugateofstreptavidiniscommonlyusedtogetherwithaconjugateofbiotinforspecificdetectionofavarietyofproteins,proteinmotifs,nucleicacids,andothermolecules(forexample,abiotinylatedprimaryantibodyboundtoaproteintargetcanbedetectedwithafluorescentlylabeledstreptavidin).Strategiessimilartothisareusedinmanydetectionprotocolsincludingwesternblots,flowcytometry,imagingandmicroscopy,andmicroplateassays,andarealsousedinpurificationworkflowstoachievetargetedfractionation.Qdot®nanocrystalconjugatesareprovidedas1µMsolutions.
ImportantFeaturesofQdot®StreptavidinConjugates:
•StreptavidinQdot®625conjugatehasemissionmaximaof~625nm
•Approximately5to10streptavidinsperQdot®nanocrystal
•Extremelyphotostableandbrightfluorescence
•Efficientlyexcitedwithsingle-lineexcitationsources
•Narrowemission,largeStokesshift
•Availableinmultiplecolors
•Idealforwesternblots,flowcytometry,imagingandmicroscopy,microplateassaysandmore
PropertiesofQdot®Nanocrystals
TheQdot®streptavidinconjugateisthesizeofalargemacromoleculeorprotein(~15–20nm)andrepresentsthebrightestclassofstreptavidindetectionreagents.Qdot®streptavidinconjugatesaremadefromananometer-scalecrystalofasemiconductormaterial(CdSe),whichiscoatedwithanadditionalsemiconductorshell(ZnS)toimprovetheopticalpropertiesofthematerial.TheQdot®705andQdot®800streptavidinconjugates,whichincludeCdSeTe,aremadeinasimilarfashion.Thiscore-shellmaterialisfurthercoatedwithapolymershellthatallowsthematerialstobeconjugatedtoBIOLOGicalmoleculesandtoretaintheiropticalproperties.
OtherFluorescentConjugatesofStreptavidinareAvailable
WeofferseveralotherQdot®colorsortrytheQdot®StreptavidinSamplerKit,containingQdot®streptavidinconjugatesinsixcolors525,565,585,605,655,and705.Inadditiontonanocrystalconjugates,weofferawiderangeofstreptavidinsconjugatedtoAlexaFluor®dyes,OregonGreen®dye,enzymeconjugates,andtrADItionalfluorophoreslikeTexasRed®dye,fluorescein(FITC)andmore.
FindBiotinylatedConjugates
Weofferanextensivelineofbiotinylatedconjugatesforuseinbiotin-streptavidindetectionstrategies.
•Usetheprimaryantibodysearchtooltofindbiotinylatedprimaryantibodies
•Usethesecondaryantibodyselectortooltofindbiotinylatedsecondaryantibodiesandbiotinylatedanti-dyeandanti-haptenantibodies
BlockingEndogenousBiotin
Naturallyoccurringbiotinscaninterferewithbiotin-streptavidindetectionschemes.ForexperimentsinvolvingfixedandpermeABIlizedcells,tryourEndogenousBiotin-BlockingKittominimizethisinterference.
赛默飞世尔科技进入中国发展已超过35年,在中国的总部设于上海,并在北京、广州、香港、成都、沈阳、西安、南京、武汉、昆明等地设立了分公司,员工人数超过4000名。我们的产品主要包括分析仪器、实验室设备、试剂、耗材和软件等,提供实验室综合解决方案,为各行各业的客户服务。
为了满足中国市场的需求,现有6家工厂分别在上海、北京和苏州运营。在全国还设立了5个应用开发中心,将世界级的前沿技术和产品带给中国客户,并提供应用开发与培训等多项服务;位于上海的中国创新中心结合中国市场的需求和国外先进技术,研发适合中国的技术和产品;公司拥有遍布全国的维修服务网点和特别成立的中国技术培训团队,在全国有超过2000名专业人员直接为客户提供服务。