Invitrogen™C10589/CellLight™LateEndosomes-RFP现货低价
1mL
CellLight®LateEndosomes-RFP,BacMam2.0,providesaneasywaytolabellateendosomeswithredfluorescentprotein(RFP)inlivecells.Simplyaddthereagenttoyourcells,incubateovernight,andthecellsarereadytoimageinthemorning.
Wanttolabelothercellstructures?LearnmoreaboutCellLight®fluorescentproteinlabelingtools
Thisready-to-useconstructistransfectedintocellsusingBacMam2.0technology,whereitexpressesRFPfusedtoRab7a.Youcanobservelateendosomes-RFPbehaviorinlivecellsindependentlyoforganellepHandlabelwithmultipletrackingortracingdyestoimagedynamiccellularprocesses.
CellsexpressingCellLight®constructscanalsobefixedwithformaldehydeformultiplexedimagingusingimmunocytochemicaltechniques.
CellLight®Technologyis:
•Fastandconvenient:simplyaddCellLight®reagenttoyourcells,incubateovernight,andimage—orstorefrozen,assay-readycellsforlateruse
•Highlyefficient:upto90%transductionofawiderangeofmammaliancelllines,includingprimarycells,stemcells,andneurons
•FlexIBLe:co-transducemorethanoneBacMamreagentformultiplexexperimentsorco-localizationstudies;tightlycontrolexpressionlevelsbysimplyvaryingthedose
•Lesstoxic:CellLight®reagentsarenon-replicatinginmammaliancellsandaresuitableforbiosafetylevel(BSL)1handling
BacMamTechnology
CellLight®LateEndosomes-RFP,BacMam2.0,isafusionconstructofRab7aandTagRFP,providingaccurateandspecifictargetingtocellularLateEndosomes-RFP.Thisfusionconstructispackagedintheinsectvirusbaculovirus,whichdoesnotreplicateinhumancellsandisdesignatedassafetousewithbiosafetylevel(BSL)1inmostlaboratories.BacMamtechnologyensuresthatmostmammaliancelltypesaretransduced/transfectedwithhighefficiencyandminimaltoxicity.Thistransienttransfectioncanbedetectedafterovernightincubationforuptofivedays—enoughtimetocarryoutmostdynamiccellularanalyses.Likeanytransfection/transductiontechnique,theBacMammethoddoesnottransfect/transduceallofthecellswithequalefficiency,makingitpoorlysuitedtocellularpopulationstudiesorautomatedimaging/counting.CellLight®reagentsareidealforexperimentswherecellularorsubcellularco-locatizationisrequired,orforcellularfunctionstudiesthatneedspecialresolution.
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