欢迎广大客户咨询订货，在线QQ：3507623898（点击即可添加QQ好友咨询）
4000-520-616
Reagentproteins是临床前和cGMP级CRM197的全球领先供应商，供给多糖和半抗原的免疫原性的载体蛋白。为确保发展的科学家和制造业供应链的专业人士提供了准备进入临床前和cGMP毫克公斤级CRM197从整个产品的开发和商业化的充分供应。试剂蛋白CRM197是由生物主文件支持（BMF）向美国食品和药物管理局（FDA）药品评价和研究中心（CBER）。
CatalogNumber
CRM-197
Description
CRM197diphtheriatoxinmutant,recombinant.Pre-clinicalgrade.
ProductBackground
CRM197isanon-toxicmutantofdiphtheriatoxin1,2 havingasingleaminoacidsubstitutionofglutamicacidforglycine.CRM197isawelldefinedproteinandfunctionsasacarrierforpolysaccharidesandhaptensmakingthemimmunogenic.Itisutilizedasacarrierproteininanumberofapprovedconjugatevaccinesfordiseasessuchasmeningitisandpneumococcalbacterialinfections.PfenexCRM197isarecombinantformexpressedinPseudomonasfluorescensusingthe Pf?nexExpressionTechnologyTM platform.
Thisproductisavailableinbothpre-clinicaland cGMPgrade.
FormoreinformationonCRM197orourothervaccinecarriers,please contactus orvisitour knowledgecenter for articles, publications andotherresourcesonCRM197andourothervaccinecarriers.
Category
VaccineComponents
Source
Pseudomonasfluorescens
Species
CoryNEBacteriumdiphtheriae
Formulation
CRM197islyophilizedfroma0.2um-filteredsolutioncontaining4mg/mLCRM197,50mg/mLsucrose,0.22mg/mLsodiumphosphatemonobasic,3mg/mLsodiumphosphatedibasic,0.055mg/mLpolysorbate80.
ProductMolecularMass:58.4kDa
Storage
Storeat2-8°Cuponreceipt
Purity
Purity:>95%CRM197bySDS-PAGE
AandBchaincontent<5%oftotalCRM197
Endotoxin:<100EU/mgofproteinbyLALmethod
Dimer:<5%
Uses
Beforeuse,asepticallyreconstitutelyophilizedCRM197withsteriledeionizedwatertoaconcentrationof4mg/mLCRM197.ReconstitutedCRM197shouldbestoredat2-8°C.
References
1.Giannini,G.,Rappuoli,R.,andRatti,G.NucleicAcidsRes.12:4063-4069(1984)
2.Mekada,E.,andUchida,T.J.Biol.Chem.260:12148-12153(1985)
