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cellscript公司全系列产品清单

来者：小蚂哥

Cellscript公司全系列产品清单

Cellscript™的使命是为 临床研究和治疗提供最佳的产品和技术，用于制造和使用RNA在细胞中进行翻译。

目前的产品包括用于体外转录的 试剂盒、使用cap模拟物或cap酶的5'RNAcapping和3'RNA聚腺苷酸化的试剂盒，以及用于制造用于细胞翻译的cap、poly(a)-tailmRNA的all-in-one试剂盒。

Cellscript™最近推出了隐身™的体外合成RNA的RNA转录套件包含修饰核苷,如假尿苷(Ψ)和/或5-methylcytidine(m5C)代替相应的U或C规范核苷(图1)。这些工具都取名为“因为封顶,聚腺苷酸和核苷修饰的RNA产物(称为“INCOGNITOmRNAs”)是经过伪装的，因此当它们被转染到表达多种RNA传感器的哺乳动物细胞中时，不会像相应的未经修饰的mrna一样，引发先天免疫反应。

产品清单：

mRNAProduction

T7mScript™StandardmRNAProductionSystem (PDF)

Simplythebestwaytomake invitro mRNA.Combinesthebenefitsofhigh-yieldtranscriptionwiththoseofpost-transcriptionalcappingandtailingallinonekit.SeeT7-FlashScribeTranscriptionKit,ScriptCapm⁷GCappingSystem,ScriptCap2'-O-MethyltransferaseKitandA-PlusPoly(A)PolymeraseTailingKitonthispage.

C-MSC11610	10reactions	$320
C-MSC100625	25reactions	$702

InVitro Transcription

T7-FlashScribe™TranscriptionKit (PDF)

Fortheproductionof invitro transcribedRNAwithT7RNApolymeraseandcanonical(GAUC)NTPs.Areactionproduces180µgRNAfrom1µgDNAin
30minutes.

C-ASF3507

50reactions

$286

T7-Scribe™StandardRNAIVTKit (PDF)

Fortheproductionof invitro transcribedRNAwithT7RNApolymeraseandcanonical(GAUC)NTPs.Areactionproduces150µgRNAfrom1µgDNAin2hours.

C-AS3107

50reactions

$267

SP6-Scribe™StandardRNAIVTKit (PDF)

Fortheproductionof invitro transcribedRNAwithSP6RNApolymeraseandcanonical(GAUC)NTPs.Areactionproduces90µgRNAfrom1µgDNAin2hours.

C-AS3106

50reactions

$267

Post-TranscriptionalCapping

ScriptCap™m⁷GCappingSystem (PDF)

Forpost-transcriptionalcappingof invitro transcribedRNAwithScriptCapCappingEnzyme,GTPandSAM.Cap0(N7-methyl-G)capscanbebuiltwithefficienciesapproaching100%.Thiscannotbeaccomplishedwithco-transcriptionalcappingmethods.UseinconjunctionwithScriptCap2'-O-Methyltransferasetoproduce
Cap1caps,thatwhichisfoundonmosteukaryoticmRNAs.

C-SCCE0625

25reactions

$436

ScriptCap™2'-O-MethyltransferaseKit (PDF)

ForconversionofRNAcontainingCap0capstothosecontainingCap1caps,whicharefoundonmosteukaryoticmRNAs.CanbeusedsimultaneouslywithScriptCapm⁷GCappingSystemtoproducevirtually100%Cap1-cappedRNAinasinglereaction.

C-SCMT0625

25reactions

$219

ScriptCap™Cap1CappingSystem (PDF)

Forpost-transcriptionalcappingof invitro transcribedRNAwithScriptCapCappingEnzyme,ScriptCap2'-O-Methyltransferase,GTPandSAM.EitherCap0orCap1capscanbebuiltwithefficienciesapproaching100%.Thiscannotbeaccomplishedwithco-transcriptionalcappingmethods.

C-SCCS1710

10reactions

$211

Co-TranscriptionalCapping

MessageMAX™T7ARCA-CappedMessageTranscriptionKit (PDF)

Forco-transcriptionalcappingofIVTRNAwithT7RNApolymeraseandAnti-Reversecapanalog(ARCA).Areactionproduces60µgRNAfrom1µgDNAin30minutes.Reactionproductsare80%correctlycappedand20%uncapped.

C-MMA60710

10reactions

$323

AmpliCap-Max™T7HighYieldMessageMakerKit (PDF)

Forco-transcriptionalcappingofIVTRNAwithT7RNApolymeraseandstandardcapanalog.Areactionproduces60µgRNAfrom1µgDNAin30minutes.Reactionproductsare40%correctlycapped,40%incorrectlycappedand20%uncapped.

C-ACM04037

25reactions

$368

AmpliCap™SP6HighYieldMessageMakerKit (PDF)

Forco-transcriptionalcappingofIVTRNAwithSP6RNApolymeraseandstandardcapanalog.Areactionproduces35µgRNAfrom1µgDNAin2hours.Reactionproductsare40%correctlycapped,40%incorrectlycappedand20%uncapped.

C-AC0706

25reactions

$368

Post-TranscriptionalTailing

A-Plus™Poly(A)PolymeraseTailingKit (PDF)

Forpost-transcriptionalpoly(A)tailadditiontoRNAs.AllowstailingofmRNAs
invitro,tolengthsequivalenttoorgreaterthanthosefoundinnature.Thiscannotbeaccomplishedwithco-transcriptionaltailingmethods.

C-PAP5104H

50reactions

$219

ModifiedNTP InVitro Transcription

INCOGNITO™T7Ψ-RNATranscriptionKit (PDF)

Fortheproductionofpseudouridine-containing, invitro transcribedRNAwithT7RNApolymerase.Ψ-mRNAsinducelowerinnateimmuneresponsesinmammaliancells.Areactionproduces100µgRNAfrom1µgDNAin2hours.

C-ICTY110510

10reactions

$410

INCOGNITO™SP6Ψ-RNATranscriptionKit (PDF)

Fortheproductionofpseudouridine-containing, invitro transcribedRNAwithSP6RNApolymerase.Ψ-mRNAsinducelowerinnateimmuneresponsesinmammaliancells.Areactionproduces60µgRNAfrom1µgDNAin2hours.

C-ICSY110510

10reactions

$410

INCOGNITO™T75mC-&Ψ-RNATranscriptionKit (PDF)

Fortheproductionof5-methylcytosine-pseudouridine-containing, invitrotranscribedRNAwithT7RNApolymerase.5mC-Ψ-mRNAsinducelowerinnateimmuneresponsesinmammaliancells.Areactionproduces100µgRNAfrom1µgDNAin2hours.

C-ICTMY110510

10reactions

$691

INCOGNITO™T7ARCA5mC-&Ψ-RNATranscriptionKit (PDF)

Forco-transcriptionalcappingof5-methylcytosine-pseudouridine-containing,
invitro transcribedRNAwithT7RNApolymeraseandAnti-Reversecapanalog(ARCA).5mC-Ψ-mRNAsinducelowerinnateimmuneresponsesinmammaliancells.Areactionproduces40µgRNAfrom1µgDNAin3hours.Reactionproductsare80%correctlycappedand20%uncapped.

C-ICTAMY110510

10reactions

$842

mRNADecapping

Cap-Clip™AcidPyrophosphatase (PDF)

Fortheremovalofcapstructures(decapping)fromvariousRNAs(i.e.mRNAs)resultingin5'-monophosphateRNAs.Canbesubstitutedunitforunit,intoanypreexistingprotocolforTobaccoAcidPyrophosphatase(TAP)utilizingthesamebuffer.

C-CC15011H

100units

$550