invitrogen公司A11032抗体现货
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Goatanti-MouseIgG(H+L)HighlyCross-AdsorbedSecondaryAntibody,AlexaFluor594
ProductDetails
TestedApplications
Dilution
FlowCytometry(Flow)
1-10µg/mL
Immunocytochemistry(ICC)
2µg/mL
Immunofluorescence(IF)
2µg/mL
PublishedApplications
Immunohistochemistry(Frozen)(IHC(F))
Immunohistochemistry(IHC)
Immunocytochemistry(ICC)
Immunohistochemistry(Paraffin)(IHC(P))
MiscellaneousPubMed(MISC)
ProductSpecifications
SpeciesReactivity
Mouse
Host/Isotype
Goat/IgG
Class
Polyclonal
Type
SecondaryAntibody
Immunogen
GammaImmunoglobinsHeavyandLightchains
Conjugate
AlexaFluor®594
Excitation/EmissionProfile
Form
Liquid
Concentration
2mg/mL
Purification
purified
Storagebuffer
PBS,pH7.5
Contains
5mMsodiumazide
Storageconditions
4°C,storeindark
RRID
AB_2534091
Target
IgG
CrossAdsorption
AgainstbovineIgG,goatIgG,rabbitIgG,ratIgG,humanIgGandhumanserum
AntibodyForm
WholeAntibody
ProductSpecificInformation
Tominimizecross-reactivity,thesegoatanti-mouseIgG(H+L)wholesecondaryantibodieshavebeenaffinitypurifiedandcross-adsorbedagainstbovineIgG,goatIgG,rabbitIgG,ratIgG,humanIgG,andhumanserum.Cross-adsorptionorpre-adsorptionisapurificationsteptoincreasespecificityoftheantibodyresultinginhighersensitivityandlessbackgroundstaining.Thesecondaryantibodysolutionispassedthroughacolumnmatrixcontainingimmobilizedserumproteinsfrompotentiallycross-reactivespecies.Onlythenonspecific-bindingsecondaryantibodiesarecapturedinthecolumn,andthehighlyspecificsecondariesflowthrough.Thebenefitsofthisextrastepareapparentinmultiplexing/multicolor-stainingexperiments(e.g.,flowcytometry)wherethereispotentialcross-reactivitywithotherprimaryantibodiesorintissue/cellfluorescentstainingexperimentswheretherearemaybethepresenceofendogenousimmunoglobulins.
AlexaFluordyesareamongthemosttrustedfluorescentdyesavailabletoday.Invitrogen™AlexaFluor594dyeisabright,red-fluorescentdyewithexcitationideallysuitedtothe594nmlaserline.Forstablesignalgenerationinimagingandflowcytometry,AlexaFluor594dyeispH-insensitiveoverawidemolarrange.Probeswithhighfluorescencequantumyieldandhighphotostabilityallowdetectionoflow-abundancebiologicalstructureswithgreatsensitivity.AlexaFluor594dyemoleculescanbeattachedtoproteinsathighmolarratioswithoutsignificantself-quenching,enablingbrighterconjugatesandmoresensitivedetection.Thedegreeoflabelingforeachconjugateistypically2-8fluorophoremoleculesperIgGmolecule;theexactdegreeoflabelingisindicatedonthecertificateofanalysisforeachproductlot.
Usingconjugatesolutions:Centrifugetheproteinconjugatesolutionbrieflyinamicrocentrifugebeforeuse;addonlythesupernatanttotheexperiment.Thisstepwillhelpeliminateanyproteinaggregatesthatmayhaveformedduringstorage,therebyreducingnonspecificbackgroundstaining.Becausestainingprotocolsvarywithapplication,theappropriatedilutionofantibodyshouldbedeterminedempirically.Forthefluorophore-labeledantibodiesafinalconcentrationof1-10µg/mLshouldbesatisfactoryformostimmunohistochemistryandflowcytometryapplications.
Background/TargetInformation
WeofferanextensivelineofInvitrogen™secondaryantibodyconjugateswithwell-characterizedspecificityandlabeledwithawideselectionofpremiumfluorescentdyes,includingInvitrogen™AlexaFluor™fluorescentdyes.Fluorescentsecondaryantibodyconjugatesareusefulinthedetection,sorting,orpurificationofitsspecifiedtargetandidealforfluorescencemicroscopyandconfocallaserscanningmicroscopy,flowcytometry,andfluorescentwesterndetection.Thebreadthoffluorescent Markersweofferallowsourreagentstobetailoredtoalmostanyfluorescentdetectionsystem.
Secondaryantibodiesmaybeprovidedinthreeformats:wholeIgG,divalentF(ab')2fragments,andmonovalentFabfragments.Becauseofthehighdegreeofconservationinthestructureofmanyimmunoglobulindomains,mostclass-specificsecondaryantibodiesmustbeaffinity-purifiedandcross-adsorbedtoachieveminimalcross-reactionwithotherimmunoglobulins.
Oursecondaryantibodyconjugatesaremostcommonlypreparedbyimmunizingthehostanimalwithapooledpopulationofimmunoglobulinsfromthetargetspeciesandcanbefurtherpurifiedandmodified(e.g.,immunoaffinitychromatography,antibodyfragmentation,labelconjugation,etc.)togeneratehighlyspecificreagents.Inthefirstroundofpurification,wholeimmunoglobulinsbindingtotheimmunizingantibodyarerecoveredandmainlyconsistofthe~150-kDaIgGclass.Furtherpurification,forexample,withProteinAorG,removesallunwantedimmunoglobulinclassesexcepttheaffinity-purifiedantibodiesthatreactwiththetarget-specificimmunoglobulinheavyand/orlightchains.
ForResearchUseOnly.Notforuseindiagnosticprocedures.Notforresalewithoutexpressauthorization.
